Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Glycoprotein – e.g. – mucins – proteoglycans – etc.
Patent
1993-06-17
1996-01-23
Wax, Robert A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Glycoprotein, e.g., mucins, proteoglycans, etc.
435 691, 435 697, 4352523, 4353201, 536 234, 536 235, 935 10, 935 47, 935 50, 935 70, C07K 14435, C07K 1900, C12N 1512, C12N 1562
Patent
active
054865999
ABSTRACT:
A purified mammalian proteoglycan, and genetic information encoding such proteoglycans, having a core polypetide molecular weight of about 30 kD to about 35 kD, and comprising a hydrophilic amino terminal extracellular region, a hydrophilic carboxy terminal cytoplasmic region, a transmembrane hydrophobic region between said cytoplasmic and extracellular regions, a protease susceptible cleavage sequence extracellularly adjacent the transmembrane region of the peptide, and at least one glycosylation site for attachment of a heparan sulfate chain to said extracellular region, said glycosylation site comprising a heparan sulfate attachment sequence represented by a formula Xac-Z-Ser-Gly-Ser-Gly, where Xac represents an amino acid residue having an acidic sidechain, and Z represents from 1 to 10 amino acid residues. Additional peptides having this glycosylation site and genetic information useful for preparing a number of variations based on this glycosylation site are also provided.
REFERENCES:
patent: 4496545 (1985-01-01), Scherschlicht et al.
patent: 4840941 (1989-06-01), Ueno et al.
patent: 4945086 (1990-07-01), Benitz et al.
patent: 4956347 (1990-09-01), Ban et al.
patent: 5075227 (1991-12-01), Hagen
patent: 5169628 (1992-12-01), Wathen
patent: 5180808 (1993-01-01), Ruoslahti et al.
patent: 5180809 (1993-01-01), Ruoslahti et al.
patent: 5198423 (1993-03-01), Taguchi et al.
patent: 5211657 (1993-05-01), Yamada et al.
Bilozur et al; J. Biol. Chem. 265(32):19697-19703 (Nov. 15, 1990).
Kiefer et al.; PNAS 87:6985-6989 (Sep. 9, 1990).
Nakamura et al; J. Biol. Chem. 266(29):19432-19437 (Oct. 15, 1990).
Bernfield et al., "Syndecan, a Development regulated Cell . . . ", Phil. Trans. R. Soc. Lond. (1990) 327:171-186.
Sanderson et al., "Molecular Polymorphism of a Cell Surface . . . ", PNAS (1989) 85:9562-9566.
Saunders et al., "Cell Surface Proteoglycan Binds Mouse . . . ", J. Cell Bio. (1988) 106:423-430.
Koda et al., "Heparan Sulfate Proteoglycans from Mouse . . . ", J. Biol. Chem. (1985) 260:8157-8162.
Rapraeger et al. "Cell Surface Proteoglycan of Mammary . . . ", J. Biol. Chem. (1985) 260:4103-4109.
Fransson; Carbohydrate Research 62:235-244 (1978).
Fransson et al; Biochem. J. 175:299-309 (1978).
Gamse et al; Biochim. Biophys. Acta 544:514-528 (1978).
Ayotte et al.; Carb. Res. 145:267-2377 (1986).
Linhardt et al; Biochem. 29(10):2611-2617 (Mar 1990).
Casu; Adv. Carb. Chem. Biochem. 43: 117-119 (1985).
Ross et al., "Purification of a basic fibroblast gorwth factor-binding proteoglycan from bovine cardiac plasma membrane" Biochimica et Biophysica Acta 1993, vol. 1145, pp. 219-226.
Bernfield et al., "Biology of the Syndecans: A Family of Transmembrane Heparan Sulfate Proteoglycans" Ann. Rev. Cell Biol. 1992, vol. 8, pp. 365-393.
Carey et al., "Molecular Cloning and Characterization of N-Syndecan, a Novel Transmembrane Heparan Sulfate Proteoglycan" The Journal of Cell Biology 1992, vol. 117, No. 1, pp. 191-201.
Cizmeci-Smith, et al., "Regulated Expression of Syndecan in Vascular Smooth Muscle Cells and Cloning of Rat Syndecan Core Protein cDNA" The Journal of Biological Chemistry 1992, vol. 267, No. 22, pp. 15729-15736.
David et al., "Molecular Cloning of Amphiglycan, a Novel Integral Membrane Heparan Sulfate Proteoglycan Expressed by Epithelial and Fibroblastic Cells" The Journal of Cell Biology 1992, vol. 118, No.4, pp. 961-969.
Gould et al., "Syndecan 3: A member of the syndecan family of membrane-intercalated proteoglycans that is expressed in high amounts at the onset of chicken limb cartilage differentiation" PNAS 1992, vol. 89, pp. 3271-3275.
Hardingham et al., "Proteoglycans: many forms and many functions" FASEB Journal 1992, vol. 6, pp. 861-870.
Ishihara et al., "A Cell-Based Assay for Evaluating the Interaction of Heparin-like Molecules and Basic Fibroblast Growth Factor" Analytical Biochemistry 1992, vol. 202, pp. 310-315.
Kojima et al., "Molecular Cloning and Expression of Two Distinct cDNA-encoding Heparan Sulfate Proteoglycan Core Proteins from a Rat Endothelial Cell Line" The Journal of Biological Chemistry 1992, vol. 267, No. 7, 4870-4877.
Lories et al., "Differential Expression of Cell Surface Heparan Sulfate Proteoglycans in Human Mammary Epithelial Cells and Lung Fibroblasts" The Journal of Biological Chemistry 1992, vol. 267, No. 2, pp. 1116-1122.
Mertens et al., "Cell Surface Heparan Sulfate Proteoglycans from Human Vascular Endothelial Cells" The Journal of Biological Chemistry 1992, vol. 267, No. 28, pp. 20435-20443.
Olwin et al., "Repression of Myogenic Differentation by aFGF, bFGF, and K-FGF is Dependent on Cellular Heparan Sulfate" The Journal of Cell Biology 1992, vol. 118, No. 3, pp. 631-639.
Pierce et al., "Molecular Cloning of the Major Cell Surface Heparan Sulfate Proteolgycan from Rat Liver" The Journal of Biological Chemistry 1992, vol. 267, No. 6, pp. 3894-3900.
Salmivirta et al., "Basic Fibroblast Growth Factor-Syndecan Complex at Cell Surface or Immobilized to Matrix Promotes Cell Growth" The Journal of Biological Chemistry 1992, vol. 267, No. 25, pp. 17606-17610.
Sanderson et al., "Syndecan-1, a Cell-Surface Proteoglycan, Changes in Size and Abundance when Keratinocytes Stratify" The Journal of Investigative Dermatology 1992, vol. 99, No. 4, pp. 390-396.
Mali et al., "Sequence of Human Syndecan Indicates a Novel Gene Family of Integral Membrane Proteoglycans" The Journal of Biological Chemistry 1990, vol. 265, No. 12, pp. 6884-6889.
Jalkanen et al., "Mouse Mammary Epithelial Cells Produce Basement Membrane and Cell Surface Heparan Sulfate Proteoglycans Containing Distinct Core Proteins" The Journal of Cell Biology 1988, vol. 106, pp. 953-962.
Jalkanen et al., "Cell Surface Proteoglycan of Mouse Mammary Epthelial Cell is Shed by Cleavage of its Matrix-binding Ectodomain from Its Membrane-associated Domain" The Journal of Cell Biology 1987, vol. 105, No. 6, Pt 2, pp. 3087-3096.
Rapraeger, A. C., et al., 1983, The Journal of Biological Chemistry, 258(6):3632-3636.
Landau, N. R., et al., 1984, Proceedings of the National Academy of Sciences, U.S.A., 81:5836-5840.
Sakselva, O., et al., 1988, The Journal of Cell Biology, 107:743-751.
Fransson, L. A., 1987, Trends in Biological Sciences, 72:408-411.
Hayashi, K., et al., 1988, Laboratory Investigation, 58(1):68-76.
Hewick, R. M., et al., 1981, The Journal of Biological Chemistry, 256(15):7990-7997.
Xu, Q.-Y., et al., 1988, Analytical Biochemistry 179(1):19-30.
Zimmerman, D. R., et al., 1989, The EMBO Journal, 8(10):2975-2981.
Weitzhandler, M., et al., 1988, The Journal of Biological Chemistry, 263(5):6949-6952.
Rapraeger, A., et al., 1985, The Journal of Biological Chemistry, 260(20):11046-11052.
Saunders, S., et al., 1989, the Journal of Cell Biology, 108:1547-1556.
Rasmussen, S., et al., 1988, The Journal of Cell Biology, 107:1959-1967.
Bourdon, M. A., et al., 1987, Proceedings of the National Academy of Sciences, U.S.A., 84:3194-3198.
Marynen, P., et al., 1989, The Journal of Biological Chemistry, 264(12):7017-7024.
Bernfield Merton
Kato Masato
Saunders Scott
Children's Medical Center Corporation
Engellenner Thomas J.
Moore William W.
The Board of Trustees of the Leland Stanford Junior University
Vincent Matthew P.
LandOfFree
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