Construction and use of functional minichromosomes

Details Construction and use of functional minichromosomes Construction and use of functional minichromosomes

1982-12-22

1984-08-07

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

435 68, 435 70, 435 91, C12N 116, C12P 2100, C12P 2102, C12P 1934

Patent

active

044644728

ABSTRACT:
Functioning eukaryotic minichromosomes containing a centromere, at least one replicating site, and at least one functioning structural gene. Centromeres are obtained from a host chromosome and used as a genetic unit in combination with an autonomously replicating segment for high frequency stable transformation of host and other eukaryotic cells. The transformed eukaryotic cells can be cultivated through repeated passages without significant loss of the minichromosome and continued expression of the structural genes present in the minichromosome.

REFERENCES:
Ruddle et al.: Nature 294, 115, (1981).
Lewin: Gene Expression, vol. 2, second edition, John Wiley & Sons, New York, 1980, pp. 242-252.
Klobutcher et al.: Nature 280, 657, (1979).
Hinnen et al.: Proc. Natl. Acad. Sci., USA: 75, 1929, (1978).
Struhl et al.: Proc. Natl. Acad. Sci., USA: 76, 1035, (1979).

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