Constructing a high density cell culture system

Plastic and nonmetallic article shaping or treating: processes – Direct application of electrical or wave energy to work – Producing or treating porous product

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21912167, 264126, 264154, 264482, 4251744, B29C 6720, B23K 2610

Patent

active

055891128

ABSTRACT:
An annular culture vessel for growing mammalian cells is constructed in a one piece integral and annular configuration with an open end which is closed by an endcap. The culture vessel is rotatable about a horizontal axis by use of conventional roller systems commonly used in culture laboratories. The end wall of the endcap has tapered access ports to frictionally and sealingly receive the ends of hypodermic syringes. The syringes permit the introduction of fresh nutrient and withdrawal of spent nutrients. The walls are made of conventional polymeric cell culture material and are subjected to neutron bombardment to form minute gas permeable perforations in the walls.

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