Constitutively active phosphatidylinositol 3-kinase and uses the

Details Constitutively active phosphatidylinositol 3-kinase and uses the Constitutively active phosphatidylinositol 3-kinase and uses the

1995-02-17

2000-03-28

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing organic compound containing a metal or atom other...

435194, 435 6, 435 15, 435975, 536 232, C12N 912, C12N 1554, C12P 900

Patent

active

060430621

ABSTRACT:
The invention provides a method of producing a constitutively active phosphatidylinositol 3-kinase (PI 3-kinase) comprising the catalytic p110 subunit covalently attached at the N-terminus to the iSH2 region of the regulatory subunit, p85. The invention discloses one form of the constitutively active kinase, p110*, which functions independently of growth factor stimulation. Expression vectors encoding a constitutively active PI 3-kinase and cells containing such expression vectors are provided. The invention also provides methods of using the constitutively active phosphatidylinositol 3-kinase to generate phosphoinositides, to identify cellular target proteins and associating molecules of PI 3-kinase, to screen for inhibitors of PI 3-kinase activity and to treat certain diseases, in particular, proliferative diseases. Kits comprising the constitutively active kinase are also provided.

REFERENCES:
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