Constitutively active CXCR3 G protein-coupled chemokine...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007210, C435S069100, C435S069700, C435S252300, C435S320100, C530S350000, C536S023400, C536S023500

Reexamination Certificate

active

07427487

ABSTRACT:
The invention disclosed in this patent document relates to transmembrane receptors, particularly to a human G protein-coupled receptor, more particularly to a CXC chemokine receptor subtype 3 (CXCR3), and most particularly to mutated (non-endogenous) version of the human CXCR3 for evidence of constitutive activity and uses thereof. In some embodiments the altered versions of CXCR3 are used, inter alia, for the direct identification of candidate compounds such as receptor agonists, inverse agonists, partial agonist or antagonist for use in, for example and not limitation, graft rejection; inflammatory skin disease; inflammatory bowel disease; allergic inflammation, allergic pulmonary inflammation, inflammatory demyelinating neuropathy, CNS inflammation; rheumatoid arthritis, bronchiolitis obliterans syndrome, periodontal disease and neurodegenerative disease.

REFERENCES:
patent: 6184358 (2001-02-01), Loetscher et al.
patent: 6555339 (2003-04-01), Liaw et al.
patent: 2003/0018182 (2003-01-01), Behan et al.
Rasmussen et al., Mutation of a Highly Conserved Asparatic Acid in the beta2 Adrenergic Receptor: Constitutive Activation, Structural Instability, and Conformational Rearrangement of Transmembrane Segment 6, Feb. 1999, Mol. Pharmoc. 56:175-184.

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