Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1992-04-24
1994-05-31
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435262, 4352625, 435264, 435261, 210610, 210611, C12N 120, D06M 1600, C02F 300
Patent
active
053169409
ABSTRACT:
Selection of mutant methanotrophic bacteria capable of efficiently degrading halogenated hydrocarbons typically found in numerous wastewater sources is described. The mutants are distinguishable from parental strains in having a unique resistance to the presence of copper while exhibiting unusually high degradation rates toward trichlorethylene. Methylosinus trichosporium A.T.C.C. 55314 strains are particularly good sources of the described mutants which may be obtained using a new method of selection and screening. The disclosed microorganisms may be immobilized on various matrices and are particularly adaptable for use in bioreactors. Further, the methanotrophic bacteria have antibiotic resistance to streptomycin or rifampicin B.
REFERENCES:
patent: 4269940 (1981-05-01), Patel et al.
patent: 4587216 (1986-05-01), Patel et al.
patent: 4954258 (1990-09-01), Little
patent: 5013665 (1991-05-01), Suzuki et al.
Bedard, C. and Knowles, R. (1989) "Physiology, Biochemistry and Specific Inhibitors of CH.sub.4 NH.sub.4.sup.+ and CO Oxidation by Methanotrophs and Nitrifiers" Microbiol. Reviews 53(1):68-84.
Bowman, J. P., Sly, L. I., and Hayward, A. C. (1990) "Patterns of Tolerance to Heavy Metals Among Methane-Utilizing Bacteria", Letters in Applied Microbiology, 10:85-87.
Bradford, M. M. (1976) "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding", Anal. Biochem. 72:248-252.
Brusseau, Gregory A., Tsien, Hsien-Chyang, Hanson, Richard S., and Wackett, Lawrence P. (1990) "Optimization of Trichloroethylene Oxidation by Methanotrophs and the Use of a Colorimetric Assay to Detect Soluble Methane Monooxygenase Activity", Biodegradation, 1:19-29.
Burrows, K. J., Cornish, A., Scott, D. and I. J. Higgins (1984) "Substrate Specificities of the Soluble and Particular Methane Monooxygenases of Methylosinus trichosporium OB3b", J. Gen. Microbiol., 130:3327-3333.
Cardy, D. L. N., Laidler, V., Salmond, G. P. C., and Murrell, J. C. (1991) "Molecular Analysis of the Methane Monooxygenase (MMO) Gene Cluster of Methylosinus trichosporium OB3b". Molecular Microbiology 5(2):335-342.
Carlsen, H. N., Joergensen, L. and Degn, H. (1991) "Inhibition by Ammonia of Methane Utilization in Methylococcus capsulatus (Bath)" Appl. Microbiol. Biotechnol. 35:124-127.
Cornish, A., Nicholls, K. M., Scott, P., Hunter, B. K., Aston, W. J., Higgins, I. J., and J. K. M Sanders "In vivo .sup.13 C NMR Investigations of Methanol Oxidation by the Obligate Methanotroph Methylosinus trichosporium OB3b", J. Gen. Microbiol., 130:2565-2575.
Dalton, H., Prior, S. D., Leak, D. J., and Stanley, S. H. (1984) "Regulation and Control of Methane Monooxygenase", In: Microbial Growth of C1 Compounds, R. L. Crawford and F. S. Hanson, editors.
Davis, K. I., Cornish, A. and Higgins, I. J. (1987) "Regulation of the Intracellular Location of Methane Mono-Oxygenase During Growth of Methylosinus trichosporium OB3b on methanol", J. Gen. Microbiol. 133:291-297.
De Flora, S., Zanacchi, P. Camoirano, A., Bennicelli, C. and Badolati, G. S. (1984) "Genotoxic Activity and Potency of 135 Compounds in the Ames Reversion Test and in a Bacterial DNA-Repair Test", Mutation Res. 133:161-198.
Ensley, B. D. (1991) "Biochemical Diversity of Trichloroethylene Metabolism", Annu. Rev. Microbiol., 45:283-299.
Fan, A. M. (1988) "Trichloroethylene: Water Contamination and Health Risk Assessment," Rev. Env. Contam. Toxicol, 101:55-92.
Fliermans, C. B., Phelps, T. J., Ringelberg, D., Mikell, A. T., and D. C. White (1988) "Mineralization of Trichloroethylene by Heterotrophic Enrichment Cultures", Appl. Env. Microbiol., 51(7):1709-1714.
Forstner, U., and G. T. W. Wittman (1979) Metal Pollution in the Aquatic Environment, Springer-Verlag. p. 356.
Green, T. (1983) "The Metabolic Activation of Dichloromethane and Chlorofluoromethane in a Bacterial Mutation Assay Using Salmonella typhimurium", Mutation Res., 118:277-288.
Hall, Barry G. (1991) "Increased Rates of Advantageous Mutations in Response to Environmental Challenges", ASM News, 57(2):82-86.
Hansen, R. S., Tsien, H. C., Brusseau, G. A. and Wackett, L. P. (1990) "Biodegradation of Low-Molecular-Weight Halogenated Hydrocarbons by Methanotrophic Bacteria", FEMS Microbiol. Lett., 87:273-278.
Henry, Susan M., and Grbic-Galie, Dunja (1990) "Effect of Mineral Media on Trichloroethylene Oxidation by Aquifer Methanotrophs", (1990) 20:151-169.
Infante, P. F. and Tsongas, T. A. (1987) "Mutagenic and Oncogenic Effects of Chloromethanes, Chloroethanes, and Halogenated Analogues of Vinyl Chloride," Env. Sci. Res., 25:301-327.
Jongen, W. M. F., Alink, G. M., and Koeman, J. H. (1978) "Mutagenic Effect of Dichloromethane on Salmonella typhimurium", Mutation Res., 56:245-248.
Laemmli, U. K. (1970) "Cleavage of Structural Proteins During the Assembly of the Head of the Bacteriophage T4", Nature (London) 277:680-685.
Little, C. Dane, Palumbo, Anthony V., Herbes, Stephen E., Lidstrom, Mary E., Tyndall, Richard L., and Gilmer, Penny J. (1988) "Trichloroethylene Biodegradation by a Methane-Oxidizing Bacterium", Appl. Environ. Microbiol., 54(4):951-956.
McPheat, W. L., Mann, N. H., and H. Dalton (1987) "Isolation of Mutants of the Obligate Methanotroph Methylomonas albus Defective in Growth on Methane", Arch. Microbiol., 148:40-43.
Nicolaidis, A. A., and A. W. Sargent (1987) "Isolation of Methane Monooxygenase-Deficient Mutants from Methylosinus trichosporium OB3b using Dichloromethane", FEMS Microbiol. Lett., 41:47-52.
Oldenhuis, R., Vink, R. L. J. M., Jannsen, D. B., and Witholt, B. (1989) "Degradation of Chlorinated Aliphatic hydrocarbons by Methylosinus trichosporium OB3b Expressing Soluble Methane Monooxygenase", Appl. Envir. Microbiol., 55(11):2819-2826.
Oldenhuis, R., Oedzes, J. Y., Van Der Waarde, J. J., and Janssen, D. B. (1991) "Kinetics of Chlorinated Hydrocarbon Degradation by Methylosinus trichosporium OB3b and Toxicity of Trichlorethylene", Appl. Envir. Microbiol., 57(1):7-14.
Parsons, F., Wood, P. R. and DeMarco (1984) "Transformations of Tetrachloroethylene and Trichloroethylene in Microcosms and Groundwater", J. Am. Water Works Assoc., 76:56-59.
Patel, R. N., Hou, C. T., Laskin, A. I., and Felix, A. (1982) "Microbial Oxidation of Hydrocarbons: Properties of a Soluble Methane Monooxygenase from a Facultative Methane-Utilizing Organism, Methylobacterium sp. Strain CRL-26", Appl. Envir. Microbiol., 44(5):1130-1137.
Prior, Stephen D., and Dalton, Howard (1985) "The Effect of Copper Ions on Membrane Content and Methane Monooxygenase Activity in Methanol-Grown Cells of Methylococcus capsulatus (Bath)", Journal of General Microbiology, 131:155-163.
Rittmann, B. E. and McCarty, P. L. (1981) "Substrate Flux into Biofilms of Any Thickness", J. Environ. Engrg. ASCE 107: 831-849.
Stanley, S. H., Prior, S. D., Leak, D. J. and Dalton, H. (1983) "Copper Stress Underlies the Fundamental Change in Intracellular Location of Methane Mono-Oxygenase in Methane-Oxidizing Organisms: Studies in Batch and Continuous Cultures", Biotechnol. Lett., 5(7):487-492.
Trevors, J. T., Mayfield, C. I., and Inniss, W. E. (1982) "Measurement of Electron Transport System (ETS) Activity in Soil," Microbial Ecology 8:163-168.
Tsien, H.-C. and Hanson, R. S. (1992) "Soluble Methane Monooxygenase Component B Gene Probe for Identification of Methanotrophs That Rapidly Degrade Trichloroethylene", Appl. Env. Microbiol. 58(3):953-960.
Tsien, H.-C., Brusseau, Gregory A., Hanson, Richard S., and Wackett, Lawrence P. (1989) "Biodegradation of Trichloroethylene by Methylosinus trichosporium OB3b", Appl Env. Microbiol. 55(12):3155-3161.
Wilson, J. T., and B. H. Wilson (1985) "Biotransformation of Trichloroethylene in Soil," App. Env. Microbiol. 49:242-243.
Dialog Search Report.
Georgiou George
Phelps Patricia
Speitel, Jr. Gerald E.
Board of Regents , The University of Texas System
Robinson Douglas W.
Ware Deborah K.
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