Constitutive pseudohyphal growth yeast mutants

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4351723, 4352542, C12N 119, C12N 1500, C12N 118

Patent

active

058587659

ABSTRACT:
An isolated gene and mutations thereof capable of imparting constitutive pseudohyphal growth to S. cerevisiae is provided. The isolated wild type gene referred to as ELM1 is also capable of coding for a novel protein kinase that determines the yeast morphology and specific physiological properties.

REFERENCES:
J. Rine et al., "Saccharomyces cerevisiae as a Paradigm for Modern Molecular Genetics of fungi" in Gene Manipulations in Fungi; J.W. Bennet et al., Eds.; Academic Press: San Diego, CA; pp. 25-151; 1985.
J. Chant et al., "Genetic Control of Bud Site Selection in Yeast by a Set of Gene Products That Constitute a Morphogenetic Pathway, "Cell, 65, 1203-1212 (Jun. 28, 1991).
Current Protocols in Molecular Biology; F. Ausubel et al., Eds.; John Wiley and Sons: New York, NY (1989) --Title page, Copyright page, and Contents pages (pp. iii-x).
J. Devereux et al., "A comprehensive set of sequence analysis programs for the VAX," Nucleic Acids Res., 12(1), 387-395 (1984).
D. Freifelder, "Bud Position in Saccharomyces Cerevisiae," J. Bacteriol., 80, 567-568 (1960).
C.J. Gimeno et al., "Unipolar Cell Divisions in the Yeast S. Cerevisiae Lead to Filamentous Growth: Regulation by Starvation and RAS," Cell, 68, 1077-1090 (Mar. 20, 1992).
B. Goud et al., "A GTP-Binding Protein Required for Secretion Rapidly Associates with Secretory Vesicles and the Plasma Membrane in Yeast," Cell, 53, 753-768 (1988).
M. Grenson et al., "Multiplicity of the Amino Acid Permeases in Saccharomyces Cerevisiae," Biochim. Biophys. Acta, 127, 325-338 (1966).
S.K. Hanks et al., "The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic Domains," Science, 241, 42-51 (Jul. 1, 1988).
L.H. Hartwell, "Genetic Control of the Cell Division Cycle in Yeast, IV. Genes Controlling Bud Emergence and Cytokinesis," Exptl. Cell Res., 69 265-276 (1971).
A.M. Healy et al., "CDC55, a Saccharomyces cerevisiae Gene Involved in Cellular Morphogenesis: Identification, Characterization, and Homology to the B Subunit of Mammalian Type 2A Protein Phosphatase," Mol. Cell. Biol., 11(11), 5767-5780 (Nov. 1991).
J.E. Hill et al., "Yeast/E. coli Shuttle Vectors with Multiple Unique Restriction Sites," Yeast, 2, 163-167 (1986).
A.T. Locrincz et al., "Primary structure homology between the product of yeast cell division control gene CDC28and vertebrate oncogenes," Nature, 307 183-185 (Jan. 12, 1984).
M. Rose, et a., "Structure and function of the yeast URA3gene: expression in Escherichia coli," Gene, 29, 113-124 (1984).
R. Rothstein, "Targeting, Disruption, Replacement, and Allele Rescue: Integrative DNA Transformation in Yeast," Meth. Enzymol., 194 281-301 (1991).
J. Sambrook et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harber Laboratory: Cold Spring Harbor, NY (1989) --Title page, Copyright page, and Contents pages (pp. v-xxxii).
F. Sherman et al., Laboratory Course Manual for Methods in Yeast Genetics, Cold Spring Harbor Laboratory: Cold Spring Harbor, NY (1986) --Title page, Copyright page, and Contents pages.
A.H. Siddiqui et al., "A Regulatory Region Responsible for Proline-Specific Induction of the Yeast PUT2 Gene is Adjacent to its TATA Box," Mol. Cell Biol., 8(11), 4634-4641 (Nov. 1988).
S. Shoji et al., "Amino Acid Sequence of the Catalytic Subunit of Bovine Type II Adenosine Cyclic 3', 5'-Phosphate Dependent Protein Kinase," Biochemistry, 22(15) , 3702-3709 (1983).
E.M. Southern, "Detection of Specific Sequences Among DNA Fragments Separated by Gel Electrophoresis," J. Mol Biol., 98 503-517 (1975).
K. Struhl, "Nucleotide sequence and transcriptional mapping of the yeast pet56-his3-ded1 gene region," Nucleic Acids Res., 13(23) , 8587-8601 (1985).
A. Tzagoloff et al., "Assembly of the Mitochondrial Membrane Systems XVI. Modified Form of the ATPase Proteolipid in Oligomycin-Resistant Mutants of Saccharomyces cerevisiae," FEBS Lett., 65(3), 391-395 (Jun. 1976).
J. Vieira et al., "Production of Single-Stranded Plasmid DNA," Methods Enzymol., 153, 3-11 (1987).
J. W. Wallis et al., "A Hyper-Recombination Mutation in S. cerevisiae Identifies a Novel Eukaryotic Topoisomerase," Cell, 58, 409-419 (Jul. 28, 1989).
M.J. Blacketer et al., "Regulation of Dimorphism in Saccharomyces cerevisiae: Involvement of the Novel Protein Kinase Homolog Elmlp and Protein Phosphatase 2A", Molecular and Cellular Biology, 13 (9), 5567-5581 (1993).
A.M. Myers, "Function of the Evolutionary Conserved rho Gene Family", Grant Application with Abstract submitted to Department of Health and Human Services (Jan. 1987).
A.M. Myers, "Analysis of A G Protein-Linked Signalling Pathway that Regulates Morphological Development in the S. Cerevisiae Cell Cycle", presented at the Conference on Yeast Cell Biology, Cold Spring Harbor, NY, May 13-17, 1992.
A. Myers, "Isolation of new mutations causing elongated bud phenotypes", Poster presentation at 1991 Yeast Genetics and Molecular Biology Meeting, San Francisco, CA, May 23-27, 1991, p. 88.
Jimeno et al., Science, 257:626 (1992).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Constitutive pseudohyphal growth yeast mutants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Constitutive pseudohyphal growth yeast mutants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Constitutive pseudohyphal growth yeast mutants will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1514528

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.