Constitutive production of interleukin 2 by a T cell hybridoma

Details Constitutive production of interleukin 2 by a T cell hybridoma Constitutive production of interleukin 2 by a T cell hybridoma

1981-10-07

1984-09-25

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351722, 435240, 424177, C12P 2100, C12N 1500, C12N 500, A61K 3700

Patent

active

044736428

ABSTRACT:
A process for constitutively producing murine IL-2 from hybridoma cells generated by fusing mitogen stimulated malignant murine cells with drug sensitive murine thymoma driver cells. A fusing agent is used to fuse the two parent cells together. After fusion, the hybrid cells are cultured in vitro in a supplemented, serum containing tissue culture medium to thereby constitutively produce IL-2. The medium also includes a group of suppressing compounds which will prevent unfused driver cells from replicating, and feeder cells used to nurture the growth of competent hybrid cells.

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