Constitutive production of interleukin 2 by a T cell hybridoma

Details Constitutive production of interleukin 2 by a T cell hybridoma Constitutive production of interleukin 2 by a T cell hybridoma

1981-04-29

1983-10-04

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435172, 435240, 435948, C12P 2100, C12P 2102, C12N 1500, C12N 500, C13R 191

Patent

active

044079451

ABSTRACT:
A process for constitutively producing murine IL-2 from hybridoma cells generated by fusing nitrogen stimulated malignant murine cells with drug sensitive murine thymoma driver cells. A fusing agent is used to fuse the two parent cells together. After fusion, the hybrid cells are cultured in vitro in a supplemented, serum containing tissue culture medium to thereby constitutively produce IL-2. The medium also includes a group of suppressing compounds which will prevent unfused driver cells from replicating, and feeder cells used to nurture the growth of competent hybrid cells.

REFERENCES:
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Gillis et al., "Biochemical Characterization of Lymphocyte Regulatory Molecules-II. Purification of a Class of Rat and Human Lymphokines", 124 J. Immunol. 1954 (1980).
Gillis et al., "Biochemical and Biologic Characterization of Lymphocyte Regulatory Molecules-III. The Isolation and Phenotypic Characterization of Interleukin-2 Producing T Cell Lymphomas", 125 J. Immunol. 2570 (Dec. 1980).
Goldsby et al., "Hybrid Cell Lines with T-Cell Characteristics", 267 Nature 707 (1977).
Schrader et al., "A Con A-Stimulated T-Cell Hybridoma Releases Factors Affecting Haematopoietic Colony-Forming Cells and B-Cell Antibody Responses", 283 Nature 197 (1980).
Harwell et al., "A Concanavalin A Inducible, Interleukin-2 Producing T-Cell Hybridoma", 152 J. Exp. Med. 893 (1980).
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Gillis et al., J. Immunol. 125, 2570 (1980) in Chem., Abstr. 94:28660 (1981).

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