Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-11-07
1989-02-28
Hazel, Blondel
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4352402, 43524025, 435320, 435811, 424 856, C12P 2100, C12N 1500, C12N 500
Patent
active
048085235
ABSTRACT:
The invention concerns the production of the human fibroblast interferon, IFN-.beta.1 by cells of mammalian origin which are capable of constitutively expressing a DNA sequence encoding for IFN-.beta.1, producing the IFN and secreting it into the surrounding culture media.
A specific embodiment of the invention comprises a methotrexate resistant chinese hamster ovary cell containing one or more pSVEIF molecules. The pSVEIF molecule contains a DNA sequence encoding for human INF-.beta.1 fused about 60 base pairs downstream from SV40 early start gene. The cell is capable of constitutively expressing the sequence encoding IFN-.beta.1, producing IFN-.beta.1 glycoprotein and secreting it into the surrounding medium.
The invention also concerns methods of producing IFN-.beta. by culturing and growing the cells of the invention, and isolating and purifying the IFN-.beta.1 product.
REFERENCES:
patent: 4485017 (1984-11-01), Tan et al.
Fiers et al., Phil. Trans. R. Soc., Ford B299, pp. 29-38, 1982.
Chemical Abstracts, vol. 98, Abstract No. 1171a, 1983.
Chemical Abstracts, vol. 98, Abstract No. 120598w, 1983.
McCormick et al., Molecular and Cellular Biology, vol. 4, pp. 166-172, 1984.
Knight, Jr., Methods in Enzynology, vol. 78, pp. 417-421.
Revel Michel
Rubinstein Menachem
Hazel Blondel
White John P.
Yeda Research and Development Co. Ltd.
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