Constitutent layer harvesting from a centrifuged sample in a tub

Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...

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210787, 210789, 422 72, 422101, 422102, 436 63, 436180, G01N 118

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active

053936744

ABSTRACT:
Constituent layers are harvested from a centrifuged multi-constituent material in an evacuated glass or clear plastic tube which contains a float. When possibly contaminated materials, such as blood, are being tested, the use of an evacuated tube allows the measurements to be made without the technician being exposed to the blood. The tubes are large enough to hold approximately one ml of blood, and are filled with an inert gas at low pressure. The floats are formed with a through bore into which cell bands to be harvested will settle during centrifugation. The cell bands are stabilized by a layer of a flowable material which settles onto the plasma layer during centrifugation and forms a pellicle thereon. The cell layers to be harvested are aspirated from the float bore by way of a hypodermic needle or cannula inserted into the tube and float bore.

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