Conotoxin peptides

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 24 amino acid residues in defined sequence

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514 13, A61K 3800

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active

059690968

ABSTRACT:
Substantially pure conotoxin peptides are provided which inhibit synaptic transmissions at the neuromuscular junctions and which are useful both in vivo and in assays because they specifically target particular skeletal nAChRs to the exclusion of neuronal nAChRs. The peptides are of such length that they can be made by chemical synthesis, and the preferred peptides have formula: H-His-4Hyp-4Hyp-Cys-Cys-Leu-Tyr-Gly-Lys-Cys-Arg-Arg-Tyr-4Hyp-Gly-Cys-Ser-S er-Ala-Ser-Cys-Cys-Gln-Xaa.sub.24 -NH.sub.2 wherein Xaa.sub.24 is Arg or Gly.

REFERENCES:
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patent: 5633347 (1997-05-01), Olivera et al.
patent: 5700778 (1997-12-01), Olivera et al.
Shon, et al., "A Non-competitive Inhibitor of the Nicotinic Acetlcholine Receptor fron Conus purpurascensVenom" Biochemistry 1997, 36, 9581.
Shon, et al., "Three-Dimensional Solution Structure of .alpha.-Conotoxin MII, an .alpha..sub.3 .beta..sub.2 Neuronal Nicotinic Acetylcholine Receptor-Targeted Ligand", Reprinted from Biochemistry, vol. 36(50):15693-15700 (1997).
Advance ACS Abstract, Jul. 1, 1997, K. Shoen, et al., "Society for Neuroscience", 27th Annual Meeting, 1997.
Shon, et al., "A Noncompetitive Peptide Inhibitor of the Nicotinic Acetylcholine Receptor from Conus purpurascens Venom", Biochemistry, 36:9581-9587, 1997.

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