Conjugates of polymers and antibodies specific for T lymphocytes

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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5303896, 53038875, C07K 1600

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active

058722220

ABSTRACT:
Disclosed are conjugates including a substantially nonimmunogenic polymer backbone or microbead and binding molecules, such as Fv, Fab, or F(ab').sub.2 fragments of monoclonal antibodies or whole antibodies that are bound through their Fc carbohydrate moieties or have their Fc portion modified so that they cannot effect ADCC or complement-mediated cytolysis, and that are specific for a T cell surface antigen, such as CD3, TCR, CD4, CD8, or CD28 on T cells. The polymer or microbead is preferably made of cross-linked dextran, ficoll, latex, or agarose, and is preferably of 0.1 to 10 .mu.m in size, so that it can be suspended in fluids for in vivo applications. These conjugates can be used as adjuvants to enhance the antibody response against an administered immunogen.

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Geppert et al , 138:1660-1666, 1987, Journal of Immunology.
Williams et al , 135:2249-2255, 1985, J. Immunol.
Edgington Bio/Technology 11:1117-1119 1993.
Ellenhorn et al. J.I. 144:2840-2846 1990.
Ellenhorn et al. Science 242 :569-571 1988.
Hirsch et al. J.I. 147:2088-2093 1991.
Ceuppens et al. J.I. 137:1816-1821 1986.

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