Conjugates and assays for simultaneous detection of multiple lig

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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G01N 3353

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active

058517766

ABSTRACT:
Novel conjugates and competitive and non-competitive assays for simultaneously detecting the presence or amount of at least two target ligands capable of competing with a single conjugate for binding to at least two different ligand receptors. The invention teaches and claims binding domains coupled to a signal development element to form a conjugate where each binding domain comprises at least one ligand analogue or ligand receptor depending on assay design. The binding domains are constructed such that they function independently from one another in assays for their respective target ligands. Each binding domain may bind its respective binding partners in the assay without affecting the binding reactions of other binding domains coupled to the same signal development element.

REFERENCES:
patent: 4444878 (1984-04-01), Paulus
patent: 4868109 (1989-09-01), Lansdorp
patent: 5028535 (1991-07-01), Buechler et al.
Brennan et al (1985) Science 229, 81-83.

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