Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1991-06-10
1992-11-10
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
4351885, 5303911, 5303913, 5303915, 5303917, 5303919, 530345, 530350, 530351, 530395, 530396, 530397, 530399, 530404, 530405, 530406, 530408, 530409, 530410, 424 8591, 436537, 436547, 436501, 436544, 436546, C12N 1106, C07K 1528, C07K 1514, C07K 1518, C07K 1706
Patent
active
051622181
ABSTRACT:
Polypeptide compositions are provided having a binding site specific for a particular target ligand and further having an active functionality proximate the binding site. The active functionality may be a reporter molecule, in which case the polypeptide compositions are useful in performing assays for the target ligand. Alternatively, the active functionality may be a chemotherapeutic agent, in which case the polypeptide compositions are useful for therapeutic treatment of various diseased states. A novel method for preparing such polypeptides having active functionalities proximate their binding site comprises combining the polypeptide specific for the target ligand with an affinity label including ligand having a reactive group attached thereto. The reactive group is then covalently attached to an amino acid side chain proximate the binding site and cleaved from the substrate. The substrate is eluted, leaving a moiety of the reactive group covalently attached to the polypeptide. The active funtionality may then be attached to the moiety.
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Kim Kay K.
Nucker Christine M.
The Regents of the University of California
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