Conjugate for enzyme immuno determinations

Details Conjugate for enzyme immuno determinations Conjugate for enzyme immuno determinations

1986-07-11

1989-06-27

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 28, 435188, 435810, 436512, G01N 3353, C12Q 128, C12N 996

Patent

active

048430013

ABSTRACT:
This invention teaches a process for preparing a conjugate useful in enzyme immunoassays, and the conjugate produced by the process. The process involves oxidizing an enzyme containing a carbohydrate portion, such as peroxidase, with periodic acid or an alkali metal salt thereof in an aqueous medium followed by reduction with sodium borohydride. The oxidation and reduction step may be carried out either before or after the enzyme is coupled to the immunologically effective component of the conjugate.

REFERENCES:
patent: 4410633 (1983-10-01), Hertl et al.
patent: 4486534 (1984-12-01), Albert et al.
Nakane, P. K., and A. Kawaoi, J. of Histochem. Cytochem., vol. 22, No. 12, pp. 1084-1091 (1974).
Avrameas, S., T. Ternynck and J. L. Guesdon., Scand. J.-Immunol., vol. 8, Suppl. 7, 7-23 (1978).

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