Conductive media for electrophoresis

Details Conductive media for electrophoresis Conductive media for electrophoresis

2006-10-06

2010-10-12

Noguerola, Alex (Department: 1795)

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

C204S605000, C204S469000, C204S470000

Reexamination Certificate

active

07811437

ABSTRACT:
A series of low molarity conductive media based on non-buffering univalent cations, such as sodium chloride-sodium acetate (SCA), sodium boric acid (SB), lithium boric acid, and lithium acetate mitigate the “runaway” positive feedback heating loop produced by conventional media containing biological amine buffers and permit improved DNA electrophoresis under the conditions of low salt concentration. These media serve well in ultra-fast DNA electrophoresis and in high-resolution separations of RNA and DNA fragments.

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