Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-11-21
1999-02-23
Ketter, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
4353201, 43525233, C12P 1934
Patent
active
058742590
ABSTRACT:
A system for obtaining large amounts of a genomic DNA fragment from a bacterial artificial chromosome includes a vector that has a site into which the genomic fragment can be cloned and, flanking the site are excision mediating sites. The vector also includes, between the excision mediating sites and near one of the sites, a controllable origin of replication.
REFERENCES:
Durland, R. et al., "Mutations in the trfA Replication Gene of the Broad-Host-Range Plasmid RK2 Result in Elevated Plasmid Copy Numbers," Journal of Bacteriology 172:3859-3867.
Fang, F.C. et al., "Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria," Gene 133:1-8 (1993).
Haugan, K. et al., The Phenotypes of Temperature-Sensitive Mini-RK2 Replicons Carrying Mutations in the Replication Control Gene trfA Are Suppressed Nonspecifically by Intragenic cop Mutations,: Journal of Bacteriology 174:7026-7032 (1992).
Haugan, K. et al, "The Host Range of RK2 Minimal Replicon Copy-Up Mutants is Limited by Species-Specific Differences in the Maximum Tolerable Copy Number," Plasmid 33:27-39 (1995).
Hamilton, Carol M., "A Binary-BAC System for Plant Transformation with High-Molecular-Weight DNA", Gene, 200:107-116 (1997).
Kim, et al., "Construction and Characterization of a Human Bacterial Artificial Chromosome Library", Genomics, 34: 213-218 (1996).
Koob, et al., "Cleaving Yeast and Escherichia coli Genomes at a Single Site", Science, 250: 271-273 (1990).
Posfai, et al. "In vivo Excision and Amplification of Large Segments of the Escherichia coli Genome", Nucleic Acids Research, 22 (12): 2392-2398.
Shizuya, et al., "Cloning and Stable maintenance of 300-Kilobase-Pair Fragments of Human DNA in Escherichia coli Using an F-Factor-based Vector", Proc. Natl. Acad. Sci., 89:8794-8797.
Szybalski, Waclaw, "From the Double-Helix to Novel Approaches to the Sequencing of Large Genomes", Gene, 135: 279-290.
Wild, et al., "A Broad-Host-Range in vivo Pop-Out and Amplification System for Generating Large Quantities of 50-to 100-kb Genomic Fragments for Direct DNA sequencing", Gene, 179: 181-188 (1996).
Ketter James
Wisconsin Alumni Research Foundation
Yucel Irem
LandOfFree
Conditionally amplifiable BAC vector does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Conditionally amplifiable BAC vector, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Conditionally amplifiable BAC vector will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-305794