Conditional sterility in plants

Details Conditional sterility in plants Conditional sterility in plants

2004-06-03

2010-02-23

Fox, David T (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide encodes an inhibitory rna molecule

C800S271000, C800S274000, C800S287000, C800S303000, C800S306000, C800S317000, C800S319000, C800S320000, C800S285000, C435S194000

Reexamination Certificate

active

07667096

ABSTRACT:
The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic plant cells, plant tissue and plants which contain and express at least one antisense or interference RNA specific for a thiamine biosynthetic coding sequence or a thiamine binding protein or a thiamine-degrading protein, wherein the RNA or thiamine binding protein is expressed under the regulatory control of a transcription regulatory sequence which directs expression in male and/or female reproductive tissue. These transgenic plants are conditionally sterile; i.e., they are fertile only in the presence of exogenous thiamine. Such plants are especially appropriate for use in the seed industry or in the environment, for example, for use in revegetation of contaminated soils or phytoremediation, especially when those transgenic plants also contain and express one or more chimeric genes which confer resistance to contaminants.

REFERENCES:
patent: 5110732 (1992-05-01), Benfey et al.
patent: 5401836 (1995-03-01), Baszczynski et al.
patent: 5459252 (1995-10-01), Conkling et al.
patent: 5510471 (1996-04-01), Labrun et al.
patent: 5545545 (1996-08-01), Gengenbach et al.
patent: 5633363 (1997-05-01), Colbert et al.
patent: 5635618 (1997-06-01), Capellades et al.
patent: 5641876 (1997-06-01), McElroy et al.
patent: 5668294 (1997-09-01), Meagher et al.
patent: 5808034 (1998-09-01), Bridges et al.
patent: 5837876 (1998-11-01), Conkling et al.
patent: 5962769 (1999-10-01), Albertsen et al.
patent: 5965796 (1999-10-01), Meagher et al.
patent: 6005167 (1999-12-01), Van Tunen et al.
patent: 6172279 (2001-01-01), Bridges et al.
patent: 6372967 (2002-04-01), Mariani et al.
patent: 6384304 (2002-05-01), Quandt et al.
patent: 6506550 (2003-01-01), Fulton et al.
patent: 6512164 (2003-01-01), Famodu et al.
patent: 6518483 (2003-02-01), Bruce et al.
patent: 6518485 (2003-02-01), Connett-Porceddu et al.
patent: 2002/0157129 (2002-10-01), Perez et al.
patent: 2003/0135885 (2003-07-01), Lanahan et al.
patent: 2003/0135888 (2003-07-01), Zhu et al.
patent: 2004/0013658 (2004-01-01), Fulton et al.
patent: 2005/0198707 (2005-09-01), Meagher et al.
patent: 0628635 (1994-12-01), None
patent: 9325695 (1993-12-01), None
patent: WO 98/13503 (1998-04-01), None
patent: 9827201 (1998-06-01), None
patent: 9839462 (1998-09-01), None
patent: 9904023 (1999-01-01), None
patent: 0000623 (2000-01-01), None
patent: 0129237 (2001-04-01), None
patent: 0248335 (2002-06-01), None
patent: 03066823 (2003-08-01), None
Kandasamy et al. (Mar. 2003) “Cell Cycle-Dependent Association ofArabidopsisActin-Related Proteins AtARP4 and AtARP7 with the Nucleus,” Plant J. 33:393-348.
Kandasamy et al. (Jan. 2002) “Functional Non-Equivalency of Actin Isovariants inArabidopsis,” Mol. Biol. Cell 13:251-261.
Kandasamy et al. (1999) “The Late Pollen-Specific Actins in Angiosperms,” Plant. J. 18:681-691.
Kellermann et al. (1990) “Pyruvate decarboxylase Isozyme 1,” NCBI Accession No. PO6169.
Kim et al. (1998) “A Brassica cDNA Clone Encoding a Bifunctional Hydroxymethylpyrimidine Kinase/Thiamin-Phosphate Pyrophosphorylase Involved in thiamin Biosynthesis,” Plant Mol. Biol. 37(6):955-965.
Kursteiner et al. (Jun. 2003) “The Pyruvate decarboxylase1 Gene ofArabidopsisIs Required During Anoxia but not Other Environmental Stresses,” Plant Physiol. 132:968-978.
Lauter, F.R. (1996) “Root Specific Expression of the LeRse-1 Gene in Tomato is Induced by Exposure of the Shoot to Light,” Mol. Gen. Genet. 252:751-754.
Ledoux et al. (May 1974) “DNA-Mediated Genetic Correction of ThiaminelessArabidopsis thaliana,” Nature 249:17-21.
Li et al. (1969) “Thiamine Mutants of the crucifer,Arabidopsis,” Biochem. Genet. 3:163-170.
Machado et al. (1996) “Thi1, a Thiamine Biosynthetic Gene inArabidopsis thaliana, Complements Bacterial Defects in DNA Repair,” Plant. Mol. Biol. 31:585-593.
Machado et al. (1997) “Dual Role for the Yeast THI4 Gene in Thiamine Biosynthesis and DNA Damage Tolerance,” J. Mol. Biol. 273:114-121.
Manetti et al. (1994) “nmt2 of Fission Yeast: A Second Thiamine-Repressible Gene Co-Ordinately Regulated with nmt1,” Yeast 10:1075-1082.
Mariani et al. (Jun. 1992) “A Chimaeric Ribonuclease-Inhibitor Gene Restores Fertility to Male Sterile Plants,” Nature 357:384-387.
McDowell et al. (Feb. 1996) “Structure and Evolution of the Actin Gene Family inArabidopsis thaliana,” Genetics 142:587-602.
McDowell et al. (1996) “TheArabidopsisACT7 Actin Gene is Expressed in Rapidly Developing Tissues and Responds to Several External Stimuli.” Plant Physiol. 111:699-711.
McKinney et al. (May 2001) “Small Changes in the Regulation of oneArabidopsisProfilin Isovariant, prf1, Alter Seedling Development,” Plant Cell 13:1179-1191.
McKinney et al. (Mar. 2002) “ArabidopsisContains Ancient Classes of Differentially Expressed Actin- Related Protein Genes,” Plant. Physiol. 128:997-1007.
Meagher, R.B. (2000) “Phytoremediation of Toxic Elemental and Organic Pollutants,” Curr. Opin. Plant. Biol. 3:153-162.
Meagher et al. (2003) “TheArabidopsisCytoskeletal Genome,” In: TheArabidopsisBook, American Society of Plant Biologists, 26 pages.
Meagher et al. (1996) “Phytoremediation of Heavy Metal Pollution: Ionic and Methyl Mercury,” In; OECD Biotechnology for Water use and Conservation Workshop (Cocoyoc, Mexico: Organization for Economic Co-Operation and Development), pp. 305-321.
Moffatt et al. (1988) “Positive Selection for Male-Sterile Mutants ofArabidopsisLacking Adenine Phosphoribosyl Transferase Activity,” Plant Physiol. 86:1150-1154.
Murray et al. (1989) “Codon Usage in Plant Genes,” Nucle. Acids Res. 17:477-498.
Nishimura (Apr. 1991) “A Constitutive Thiamine Metabolism Mutation, thi80, Causing Reduced Thiamine Pyrophosphokinase Activity inSaccharomyces cerevisiae,” J. Bacteriol. 173:2716-2719.
Nishimura et al. (Jul. 1992) “A Positive Regulatory Gene, THI3, is Required for Thiamine Metabolism inSaccharomyces cerevisiae,” J. Bacteriol. 174:4701-4706.
Nishimura et al. (1993) “THI3 Regulatory Protein, (Saccharomyces cerevisiae),” NCBI Accession No. BAA04886.
Nitz et al. (2001) “Pyk10, A Seedling and Root Specific Gene and Promoter fromArabidopsis thaliana,” Plant Sci. 161:337-346.
Nosaka et al. (Aug. 1993) “Isolation and Characterization of a Thiamin Pyrophosphokinase Gene, THI80, fromSaccharomyces cerevisiae,” J. Biol. Chem. 268:17440-17447.
Nosaka et al. (1993) “Thiamine pyrophosphokinase (TPK), (Thiamine kinase),” NCBI Accession No. P35202.
Perez-Prat et al. (May 2002) “Hybrid Seed Production and the Challenge of Propagating Male-Sterile Plants,” Trends Plant. Sci. 7:199-203.
Pfahl (Jan. 1979) “Tight-Binding Repressors of the lac Operon: Selection System and in Vitro Analysis,” J. Bacteriol. 137:137-145.
Preuss et al. (Jun. 1994) “Tetrad Analysis Made Possible inArabidopsisby a Mutation of the QUARTET (QRT) Genes,” Science 264:1458-1460.
Rapala-Kozik et al. (1999) “Ligand-Protein Interaction in Plant Seed Thiamine-Binding Proteins. Binding of Various Thiamine Analogues to the Sepharose-Immobilized Buckwheat-Seed Protein,” J. Protein Chem. 18:721-728.
Redei et al. (Feb. 1969) “Effects of X-Rays and Ethyl Methanesulfonate on the Chlorophyll B Locus in the Soma and on the Thiamine Loci in the Germline ofArabidopdis,” Genetics 61:453-459.
Robb et al. (2001) “Thiamine Phosphate Pyrophosphorylase” NCBI Accession No. NP—579063.
Rugh et al. (Jul. 1998) “Toxic Mercury Reduction and Remediation Using Transgenic Plants with a Modified Bacterial Gene,” Hort. Sci. 33:12-15.
Rugh et al. (Oct. 1998) “Development of Transgenic Yellow Poplar for Mercury Phytoremediation,

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