Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids
Patent
1987-07-17
1989-09-26
Richman, Barry S.
Chemistry: analytical and immunological testing
Metal or metal containing
Present in biological fluids
204 1T, 204400, 422 68, 436 79, 436151, 436164, G01N 2746
Patent
active
048700240
ABSTRACT:
A method for determining the concentrations of total calcium and at least one monovalent ion in a sample includes the steps of mixing the sample with a diluent that has a pH within the range pH 6.5 to 7.0 and includes 2-amino-2-hydroxymethyl-1,3-propanediol phosphate and is free of the monovalent ion. An aliquot of the diluted sample is concurrently contacted with a calcium-specific ion selective electrode and an ion selective electrode specifically responsive to the monovalent ion, the response of the calcium-specific ion selective electrode is measured as an indication of the concentration of total calcium in the sample, and the response of the monovalent ion specific ion selective electrode is measured as an indication of the concentration of the monovalent ion in the sample.
REFERENCES:
patent: 4618587 (1986-10-01), Premoli
Anker, Peter, Anal. Chem. "Neutral Carrier Based Ion-Selective Electrode for the Determination of Total Calcium in Blood Serum", vol. 53, No. 13, pp. 1970-1974 (Nov. 1981).
Allied Instrumentation Laboratory, "IL Test ISE Buffer", Cat. No. 351297-10, (Mar. 1985).
Nova 7, "Total Calcium/Ionized Calcium/pH".
Bergkuist Carolyn
Manzoni Angelo
Musacchio John
Premoli Pietro
Fisher Scientific Company
Johnston Jill
Richman Barry S.
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