Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1996-03-22
1998-06-16
Gorgos, Kathryn L.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204453, 204601, 204604, G01N 2726, G01N 27447
Patent
active
057664358
ABSTRACT:
The present invention provides new methods for the concentration of ionic solutes, particularly ampholytes, such as, for example, peptides, proteins and nucleic acids. The methods are based on the fact that the electrophoretic migration velocities of solutes decrease upon a decrease in the absolute value of the zeta-potential of a solute or the pore size of the electrophoresis medium, and upon an increase in the cross-section of the electrophoresis chamber, the viscosity of the electrophoresis medium, or the electrical conductivity of the electrophoresis medium. When applied to capillary electrophoresis, the methods described herein permit concentration of a solution of solutes in the same capillary tube as is used for the electrophoretic analysis. Alternatively, however, the sample can be withdrawn from the capillary tube following concentration of the solution of solutes and processed by techniques other than high-performance capillary electrophoresis (HPCE).
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Hjerten Stellan
Liao Jia-li
Siebert Christopher
Bio-Rad Laboratories, Inc.
Gorgos Kathryn L.
Starsiak Jr. John S.
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