Concentration and size-fractionation of nucleic acids and viruse

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification

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536 254, C12N 500

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active

057078505

ABSTRACT:
The present invention relates generally to separation methods, and more particularly to a method to separate nucleic acids and viruses from other substances using porous media by combining flow rate and with electrical field strength. For example, methods to concentrate and size-fractionate of nucleic acids are hereby provided.

REFERENCES:
Alberts et al. Molecular Biology of the Cell. 1983, pp. 173-176 & 189.
Wu-Pong, "Oligonucleotides: Opportunities for Drug Therapy and Research," BioPharm, Nov. 1994, pp. 20-30.
Marquet et al., "Process Development for the Manufacture of Plasmid DNA Vectors for Use in Gene Therapy," PioPharm, Sep. 1995, pp. 26-37.
Marshall, "Gene Therapy's Growing pains," Science, vol. 269, Aug. 1995, pp. 1050-1055.
Turerk et al., "Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase," Science, vol. 249, Aug. 3, 1990, pp. 505-510.
Porath, "Column Electrophoresis on a Large Scale," Science Tools, Aug. 1964, pp. 21-27.

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