Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1980-01-08
1981-10-27
Prescott, Arthur C.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
204180G, 204180S, 424 12, G01N 2726, C25B 700
Patent
active
042971985
ABSTRACT:
In a so-called membrane electrophoresis apparatus used for separating a liquid sample containing components having biological activity such as various proteins into the components, there is provided an apparatus for simplifying the operation of applying the liquid sample on a supporting membrane and, moreover, for separating with good accuracy the above mentioned components. A supporting medium (3) for separation comprising a water-retentive membrane material and electrically connected to one electrode (5a) of a direct-current electric power source is wetted by a buffer solution (A) containing leading ions. A supporting medium (7c) and a supporting medium (9) similarly comprising water-retentive materials are respectively wetted by a buffer solution (C) containing terminating ions and a buffer solution containing leading ions and having a pH which is different from that for the above mentioned supporting medium for separation. In the state of use, after the liquid sample is applied in a linear form on the middle of the supporting medium (9), the supporting medium (9) is caused to contact at its two ends the supporting medium (3) and the supporting medium (7c) (FIG. 3), and the entire assembly assumes an electrically conductive state. The sample progressively migrates toward the electrode (5a), but because of the sharp interfaces formed by the leading ions and the terminating ions during this operation, the sample is once concentrated on the supporting medium (9) and, as it migrates toward the supporting medium (3), is separated with good accuracy into the respective components.
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Ohashi Mochihiko
Toda Tosifusa
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