Concatemeric DNA length standards

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, 4351723, 4353201, 2041828, 536 231, C12P 1934, G01N 2726

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052945456

ABSTRACT:
The use of T7 bacteriophage to produce DNA length standards by enzymatically joining terminally repetitious, blunt-ended DNA has now been demonstrated. It is now possible to precisely control the formation of concatemeric DNAs thereby generating custom-made size-ranges of length standards. Furthermore, the standards thus produced are stable over time providing a highly reproducible and convenient product for the molecular biologist.

REFERENCES:
patent: 4473452 (1984-09-01), Cantor et al.
patent: 4650761 (1987-03-01), Hershberger et al.
patent: 4710465 (1987-12-01), Weissman et al.
patent: 4784952 (1988-11-01), Ludwig et al.
patent: 5030566 (1991-07-01), Son et al.
patent: 5041203 (1991-08-01), Serwer
White, Dissertation, "Formation and Processing of Concatemers of Bacteriophage T7 DNA, In Vitro", Harvard Medical School, Harvard University, (1987).
Frohlich et al., Virology, vol. 65, 1975, pp. 455-468.
Son et al., Virology, vol. 162, 1988, pp. 38-46.
Waterbury et al., Nuc. Acids Res., vol. 15, 1987, p. 3930.
Serwer et al., Electrophorosis, vol. 8, 1987, pp. 224-246.
Ritchie et al., "Terminal Repetition in Non-permuted T3 and T7 Bacteriophage DNA Molecules," J. Mol. Biol. 23:365-376 (1967).
Kelly, T. J. and Thomas, C. A. "An Intermediate in the Replication of Bacteriophage T7 DNA Molecules," J. Mol. Biol. 44:459-475 (1969).
Kerr, C. and Sadowski, P. D. "Packaging and Maturation of DNA of Bacteriophage T7 In Vitro," Proc. Nat. Acad. Sci. USA 71:3545-3549 (Sep. 1974).
Sadowski et al., "Terminal Cross-linking of DNA Catalyzed by an Enzyme System Containing DNA Ligase, DNA Polymerase, and Exonuclease of Bacteriophage T7," Can. J. Biochem. 52:525-535 (1974).
Miller et al., "The Role of Bacteriophage T7 Exonuclease (Gene 6) in Genetic Recombination and Production of Concatemers," J. Mol. Biol. 101:223-234 (1976).
Fangman, W., "Separation of Very large DNA molecules by gel electrophoresis," Nuc. Acids Res. 5:653-665 (1978).
Kuemmerle, N. B. and Masker, W. E., "In Vitro Packaging of UV Radiation Damaged DNA from Bacteriophage T7," J. Virol. 23:509-516 (1977).
Sadowski, P. D., "Genetic Recombination of Bacteriophage T7 DNA In Vitro, II. Further Properties of the In Vitro Recombination-Packaging Reaction," Virol. 78:192-202 (1977).
Langman et al., "The structure and maturation of intermediates in bacteriophage T7 DNA replication", Can. J. Biochem 56:508-516 (1978).
Masker et al., "In Vitro Packaging of Bacteriophage T7 DNA Synthesized In Vitro", J. Virol. 27:149-163 (1978).
Rosenberg et al., "Survey and Mapping of Restriction Endonuclease Cleavage Sites in Bacteriophage T7 DNA", J. Mol. Viol. 135:907-915 (1979).
Fujisawa et al., "In Vitro Formation of the Concatemeric DNA of Bacteriophage T3 and Its Biological Activity in the in Vitro Packaging Reaction", Virol. 101:327-334 (1980).
Fujisawa et al., "Purification of DNA-Binding Proteins of Bacteriophage T3 DNA and Their Role in In Vitro Packaging of Phage T3 DNA," Virol. 105:480-489 (1980).
Yamagishi et al., "Purification of Gene 6 Product of Bacteriophage T3 and Its Role in in vitro DNA Packaging", Virol. 100:382-389 (1980).
Serwer, P., "Electrophoresis of Duplex Deoxyribonucleic Acid in Multiple-Concentration Agarose Gels: Fractionation of Molecules with Molecular Weights between 2.times.10.sup.6 and 110.times.10.sup.6 ", Biochem. 19:3001-3004 (1980).
Serwer, P. and Greenhaw, G. A., "Fractionation of Concatemeric DNA of Bacteriophage T7 by Agarose Gel Electrophoresis", Electrophoresis (1981).
Serwer, P. and Watson, R. H., "Capsid-DNA Complexes in the DNA Packaging Pathway of Bacteriophage T7: Characterization of the Capsids Bound to Monomeric and Concatemeric DNA", Virol. 198:164-176 (1981).
Dunn, J. J. and Studier, F. W., "Complete Nucleotide Sequence of Bacteriophage T7 DNA and the Locations of T7 Genetic Elements", J. Mol. Biol. 166:477-535 (1983).
Furth, M. E. and Wickner, S. H., "Lambda DNA Replication", Lambda II, pp. 145-173 (1983).
Serwer et al., "Stability and In Vitro DNA Packaging of Bacteriophages: Effects of Dextrans, Sugars, and Polyols", J. Virol. 45:665-671 (Feb. 1983).
Studier, F. W. and Dunn, J. J., "Organization and Expression of Bacteriophage T7 DNA", Cold Spring Harbor Symposia on Quantitative Biology, vol. XLVII, pp. 999-1007 (1983).
Casjens, S., "Nucleic Acid Packaging by Viruses", Virus Structure and Assembly, pp. 76-147 (1985).
Lee, D. D. and Sadowski, P. D., "In vitro concatemerization of bacteriophage T7 DNA: Role in DNA synthesis and gene 6 exonuclease", Can. J. Biochem. Cell Biol. 63:237-242 (1985).
Anand, R., "Pulsed field gel electrophoresis: a technique for fractionating large DNA molecules", Trends Gen. 2:278-283 (1986).
Hamada et al., "A Defined in Vitro System for Packaging of Bacteriophage T3 DNA", Virol. 151:119-123 (1986).
Serwer, P. and Hayes, S. J., "A voltage gradient-induced arrest of circular DNA during agarose gel electrophoresis", Electrophoresis 8:244-246 (1987).
Serwer et al., "Multidimensional Analysis of Intracellular Bacteriophage T7 DNA: Effects of Amber Mutations in Genes 3 and 19", J. Virol. 61:3499-3509 (1987).
Serwer, P., "Gel electrophoresis with discontinuous rotation of the gel: An alternative to gel electrophoresis with changing direction of the electrical field", Electrophoresis 8:301-304 (1987).
Shibata et al., "Characterization of the Bacteriophage T3 DNA Packaging Reaction in Vitro in a Defined System", J. Mol. Biol. 196:845-851 (1987).
Son, M. and Serwer, P., "The Kinetics of Bacteriophage T7 DNA Packaging in Vitro", Abstract Proc. Am. Soc. Biol. Chemists (1987).
White, J. H. and Richardson, C. C., "Processing of Concatemers of Bacteriophage T7 DNA in Vitro", J. Biol. Chem. 262:8851-8860 (1987).
White, J. H. and Richardson, C. C., "Gene 18 Protein of Bacteriophage T7, Overproduction, Purification and Characterization," J. Biol. Chem. 262:8845-8850 (1987).
Son et al., "Concatemerization and Packaging of Bacteriophage T7 DNA in Vitro: Determination of the Concatemer's Length and Appearance Kinetics by Use of Rotating Gel Electrophoresis," Virol. 162:38-46 (1988).
Watson et al., "The Replication of Bacterial Viruses", Molecular Biology of the Gene, vol. 1, 4th Ed., pp. 298-299, 503-548 (1987).
DNASTAR, The Lambda Ladder, High Molecular Weight Marker, Product Announcement Memorandum (1988).
FMC BioProducts, Size Standards for Megabase DNA, Product Announcement Memorandum (1988).
Calendar, R. (Ed.), "The Bacteriophages", vol. 1, pp. 259-289, 439-475 (date of publ. unknown).
Serwer, P. and Hayes, S. J., "A New Mode of Rotating Gel Electrophoresis For Fractionating Linear and Circular Duplex DNA: The Effects of Electrophoresis During the Gel's Rotation", Appl. Theor. Electrophoresis.

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