Computerized method and apparatus for analyzing amino acids

Details Computerized method and apparatus for analyzing amino acids Computerized method and apparatus for analyzing amino acids

2011-04-26

2011-04-26

Nagpaul, Jyoti (Department: 1773)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

C436S083000, C436S089000, C436S171000, C436S180000, C422S091000, C422S105000

Reexamination Certificate

active

07932092

ABSTRACT:
A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before β-aminoisobutyric acid (β-AiBA) is eluted.

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Kimie Murayama et al; “Resolution of 52 Ninhydrin-positive compounds with a high-speed amino acid analyzer”; Journal of Chromatography, 224 (1981) pp. 315-321; Elsevier Scientific Publishing Company-Amsterdam.
Jacques Le Boucher et al.;“Amino Acid determination in biological fluids by automated ion-exchange chromatography: performance of Hitachi L-8500A”; Clinical Chemistry 43:8, (1997) pp. 1421-1428.

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