Computer system for controlling values of operational parameters

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Cell membrane or cell surface is target

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4352851, 4351722, 935 52, 204DIG9, 204547, 205701, C12N 1300

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active

057363706

ABSTRACT:
A computer system for controlling the parameters used during molecule transfer operations. The computer system includes a processor coupled to a memory. The memory stores instructions that are executed by the processor. The computer system controls the value of parameters that affect the characteristics of pulses delivered by a pulse generating circuit to a solution. The pulse generating unit delivers individual pulses during cycles. The cumulative pulse delivered during a specified number of cycles is a pulse output. The magnitude of a pulse output is determined by the number of cycles corresponding to the pulse output and the characteristics of the individual pulses delivered during the cycles. A pulse group is a series of pulse outputs. The computer system controls a molecule transfer operation by causing the pulse generation circuit to deliver one or more pulse groups to the solution. A user selects the shape and number of pulse groups to be delivered for an operation. The computer alters the operational parameters used by the pulse generation circuit to cause the pulse generation circuit to deliver pulse groups having the selected shapes.

REFERENCES:
patent: 4622302 (1986-11-01), Sowers
patent: 4663292 (1987-05-01), Wong et al.
patent: 4849355 (1989-07-01), Wong
patent: 4946793 (1990-08-01), Marshall, III
Scangos and Ruddle, "Mechanisms and applications of DNA-mediated gene transfer in mammalian cells--a review," 14 Gene 1 (1981), pp. 1-10.
W. Anderson, "Prospects for Human Gene Therapy," 226 Science 401 (Oct. 26, 1984), pp. 401-409.
T. Wong and E. Neumann, "Electric Field Mediated Gene Transfer," 107 Biochemical and Biophysical Research Communications 584 (Jul. 30, 1982), pp. 584-587.

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