Computer logic for fluorescence genotyping at multiple allelic s

Details Computer logic for fluorescence genotyping at multiple allelic s Computer logic for fluorescence genotyping at multiple allelic s

1999-06-03

2000-11-28

Fredman, Jeffrey

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

702 19, 435 6, 435288, 2081828, G06G 758, C12Q 168

Patent

active

061547077

ABSTRACT:
A method is provided for genotyping a target sequence at at least two allelic sites by a 5' nuclease amplification reaction. In one embodiment, the method includes performing a nucleic acid amplification on a target sequence having at least two different allelic sites using a nucleic acid polymerase having 5'.fwdarw.3' nuclease activity and a primer capable of hybridizing to the target sequence in the presence of two or more sets of allelic oligonucleotide probes wherein:

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