Computer assisted biological assay system

Image analysis – Histogram processing – For setting a threshold

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435 39, 435291, G06K 900, C12Q 118

Patent

active

046370536

ABSTRACT:
The present invention is an improved system for counting of bacterial colonies on a plate. The system permits the counting of colonies under adverse colony counting conditions where there is overlap of colonies and analyzes the plate in a plurality of segments. The colony counts for each segment are subjected to count correction, size qualification and distribution qualification to correct for missed counts and to eliminate data from the system data base when the count for a segment will not produce reliable data. The entire plate is also analyzed for subsample qualification which provides the best estimate of the bacteria containing liquid which was used to produce the colonies as represented by a coefficient of variation of the bacterial density in the liquid.

REFERENCES:
patent: 3799844 (1974-03-01), Campbell et al.
patent: 3892632 (1975-07-01), Campbell et al.
patent: 3962040 (1976-06-01), Campbell et al.
patent: 4353988 (1982-10-01), Couse et al.
patent: 4517292 (1985-05-01), Schalkowsky et al.

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