Compounds that exhibit specific molecular recognition of...

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Having -c- – wherein x is chalcogen – bonded directly to...

Reexamination Certificate

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Details

C514S394000, C548S224000, C548S304700

Reexamination Certificate

active

06867227

ABSTRACT:
Asymmetric derivatives of furamidines with one of the phenyl rings of furamidine replaced with a benzimidazole have been found by quantitative footprinting analyses to bind GC containing sites on DNA more strongly than to pure AT sequences. These compounds have been shown to bind in the minor groove at specific GC containing sequences of DNA in a highly cooperative manner as a stacked dimer. Compounds of the present invention find use in selectively binding mixed sequence DNA, and may also be used in methods of regulating gene expression, methods of treating opportunistic infections and cancer, as well as in methods of detecting certain sequences of DNA.

REFERENCES:
patent: 5723288 (1998-03-01), Dykstra et al.
patent: WO 9640997 (1996-12-01), None
International Search Report for PCT/US00/34794, Apr. 27, 2001.
K.T. Hopkins et al., Extended aromatic furan derivatives as Anti-Pneumocystis cariniiAgents,Journal of Medicinal Chemistry41, 3872-3878 (1998).
D.W. Boykin et al., 2, 5-Bis[4-(N-alkylamidino)phenyl]furans as Anti-Pneumocystis cariniiAgents,J. Med. Chem. 41, 124-129 (1998).
J.L. Seifert et al., Spontaneous assembly of helical cyanine dye aggregates on DNA nanotemplates,J. Am. Chem. Soc. 121, 2987-2995 (1995).
L. Wang et al., Specific molecular recognition of mixed nucleic acid sequences: an aAromatic dication thatbinds in the DNA minor groove as a dimer,Procs. Natl. Acad. Sci. 97, 12-16 (2000).

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