Compounds for the cleavage at a specific position of RNA, oligom

Details Compounds for the cleavage at a specific position of RNA, oligom Compounds for the cleavage at a specific position of RNA, oligom

1990-02-12

1991-05-07

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, C07H 2100

Patent

active

050138308

ABSTRACT:
There is disclosed a compound having a double chain which is composed of an RNA (+chain) and a complementary DNA (-chain), wherein a portion of the DNA (-chain) has been replaced by an RNA or a derivative thereof, and wherein, when the compound is subjected to the action of an enzyme having a ribonuclease H activity, it is possible to preferentially cleave the RNA (+chain) in a position corresponding to the unsubstituted portion of the DNA (-chain). The compound can thus be used for the preferential cleavage of a phosphodiester bond in a specific position of RNA. Accordingly, the invention provides a useful means for preparing, for instance, a deletion mutant. There is also disclosed a mixed oligomer which comprises an oligomer of RNA or a derivative thereof and a DNA oligomer, wherein the RNA oligomer or a derivative thereof is conjugated to the DNA oligomer via a phosphate diester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety. There is further disclosed a nucleoside derivative of a given general formula for use as a starting material.

REFERENCES:
patent: 4401796 (1983-08-01), Itakura
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 4503151 (1985-03-01), Paddock
patent: 4661450 (1987-04-01), Kempe et al.
patent: 4725677 (1988-02-01), Koster et al.
Hideo Inoue, et al, Nucleic Acids Research, Symposium Series No. 16, 1985.
Koster, H. et al. N--acyl Protecting Groups for Deoxynucleosides. A Quantitative and Comparative Study (1981).

Affiliated with

Also associated with

Rating

Compounds for the cleavage at a specific position of RNA, oligom does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Compounds for the cleavage at a specific position of RNA, oligom, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compounds for the cleavage at a specific position of RNA, oligom will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-940597