Compounds for enzyme amplification assay methadone analogs

Details Compounds for enzyme amplification assay methadone analogs Compounds for enzyme amplification assay methadone analogs

1974-06-20

1976-06-29

Tanenholtz, Alvin E.

Textiles: fiber preparation

G01N 3114, C07G 702

Patent

active

039665569

ABSTRACT:
Novel biological assay method for determining the presence of a specific organic material by employing a modified enzyme for amplification. By employing receptors specific for one or a group of material (hereinafter referred to as "ligands") and binding an enzyme to the ligand or ligand counterfeit to provide and "enzyme-bound-ligand", an extremely sensitive method is provided for assaying for ligands. The receptor when bound to the enzyme-bound-ligand substantially inhibits enzymatic activity, providing for different catalytic efficiencies of enzyme-bound-ligand and enzyme-bound-ligand combined with receptor.
The receptor, ligand and enzyme-bound-ligand are combined in an arbitrary order and the effect of the presence of ligand on enzymatic activity determined. Various protocols may be used for assaying for enzymatic activity and relating the result to the amount of ligand present.

REFERENCES:
patent: 3850752 (1974-11-01), Schuurs et al.

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