Compounds for determining the activity of phospholipase A 2

Details Compounds for determining the activity of phospholipase A 2 Compounds for determining the activity of phospholipase A 2

2007-11-27

2007-11-27

Gitomer, Ralph (Department: 1657)

Organic compounds -- part of the class 532-570 series

Organic compounds

Phosphorus esters

C435S018000

Reexamination Certificate

active

10497672

ABSTRACT:
Compounds for determining the activity of phospholipase A2, are described herein, and include embodiments having formula (1)whereinL1is derived from an ether (R1—OR2)m, wherein R1and R2are independently selected and are derived from a hydrocarbon having 1 to 12 carbon atoms, with m being an integer from 1 to 4, or from a hydrocarbon R having 1 to 20 carbon atoms;F is unsubstituted or substituted pyrene as a flouraphore;Q is a quencher, andL2is C(O)-L1or C(O)-L1-NH, wherein L1is as defined above. These compounds may be used to determine the activity of phospholipase A2, in particular PAF-AH.

REFERENCES:
patent: 6146625 (2000-11-01), Cousens et al.
patent: 6323017 (2001-11-01), Adachi et al.
patent: 4282391 (1992-07-01), None
Hendrickson et al., “Intramolecularly Quenched BODIPY—Labeled Phosphilipid Analogs in Phospholipase A2and Platelet—Activating Factor Acetylhydrolas Assays and in Vivo Fluorescene Imaging,” Analytical Biochemistry, 276, pp. 27-35, Dec. 1, 1999.
Chihiro et al., “Preparation of phosphatidylethanolamine derivatives as substrates for phosphilipase A2determination,” Chemical Abstracts, vol. 118, No. 19, 1 page, May 10, 1993.
Bayburt et al., “Continuous, Vesicle-Based Fluorimetric Assays of 14- and 85-kDa Phosphilipases A21,” Analytical Biochemistry, 232, pp. 7-23, Nov. 20, 1995.
Thuren et al., “Phosphilipase A2 Assay Using an Intramolecularly Quenched Pyrene-Labeled Phosphilipid Analog as a Substrate,” Analytical Biochemistry, 170, pp. 248-255, Apr. 1988.

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