Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1996-02-23
1999-01-05
Low, Christopher S. F.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
514 44, 536 231, 536 235, 435 6, 435 71, 435 691, 435 911, 435 913, 4353201, 435325, 935 6, 935 62, 935 52, 935 55, 935 22, 935 23, C12N 1563
Patent
active
058561552
ABSTRACT:
A polypeptide consisting essentially of the NAB and linking region of an .alpha.-subunit of Shaker-like potassium ion channel which binds to a core region of a .beta.-subunit of said Shaker-like potassium ion channel. A related polypeptide is also provided and consists essentially of the core region of a .beta.-subunit of a Shaker-like potassium ion channel which binds to the NAB and linking region of an .alpha.-subunit of said Shaker-like channel. Nucleic acid sequences which encode these polypeptides, vectors containing those sequences, expression systems, hosts cells containing the aforesaid polypeptides, and pharmaceutical formulations of the peptides are also provided.
Other aspects of the invention include methods of modulating the flow of potassium ions through a cell membrane surrounding a cytoplasm by introducing either of the aforesaid polypeptides or exogenous Kv.beta.2 protein into the cytoplasm of the cell, methods of detecting a molecule that binds to either of the aforesaid polypeptides, and an improved yeast two-hybrid system which utilizes the aforesaid polypeptides.
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Low Christopher S. F.
Nguyen Dave Trong
The Johns Hopkins University School of Medicine
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