Compounds and methods to determine presence of Histoplasma capsu

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, 536 243, 536 2433, 536 231, C12P 1934, C12Q 168, C07H 2104, C07H 2102

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active

056935013

ABSTRACT:
The present invention combines detection and identification of H. capsulatum in one step, eliminating the need to use DNA probes to react with PCR products in order to distinguish different types of fungi. The purpose of the invention is to provide compounds and methods to directly and specifically identify H. capsulatum in clinical specimens. Specifically, we provide a unique nucleotide sequence from the rRNA gene internal transcribed spacer region I (ITSI) of H. capsulatum and a PCR method which amplifies the unique sequence of H. capsulatum.

REFERENCES:
patent: 5352579 (1994-10-01), Millman
Tisserat et al. Phytopathology 84:478-482, 1994.
LoBuglio et al. J. of Clinical Microbiology 33: 85-89, 1995.
O'Gorman et al. Canadian J. of Botany 72: 342-346, 1994.
Huffman et al. Experimental Mycology 16: 316-319, 1992.

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