Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2003-12-09
2008-12-02
Marx, Irene (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C435S018000
Reexamination Certificate
active
07459285
ABSTRACT:
Fluorogenic lysophosphatidic acid derivatives which can be used as substrates in a continuous, fluorogenic assay that can be performed in microtiter plates. The assays permit measuring LysoPLD activity levels in normal events such as pregnancy or disease states such as cancer. In addition, the present invention can be adopted to high throughout screening(HTS) for identification of potential inhibitors of lysoPLD activity.
REFERENCES:
patent: 6399392 (2002-06-01), Haugland et al.
patent: 2004/0053893 (2004-03-01), Kishimoto et al.
Hendrickson, H. Stewart, et al, “Intramolecularly Quenched BODIPY-Labeled Phospholipid Analogs in Phospholipase A2 and Platelet-Activating Factor Acetylhydrolase Assays and in Vivo Fluroescence Imaging”, Analytical Biochemistry 276, 27-35 (1999).
Aoki, J. et al, Serum Lysophosphatidic Acid is Produced Though Diverse Phospholipase Pathways,J Biol Chem, vol. 48737-44 (2002).
Bandoh, K. et al, Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species,FEBS Lett, vol. 478, 159-65 (2000).
Clair, T. et al, Autotaxin Hydrolyzes Sphingosylphosphorylcholine to Produce the Regulator of Migration, Sphingosine-1-Phosphate,Cancer Research, vol. 63, 5446-53 (2003).
Farber, S. et al, Characterization of Ca2+dependent Phospholipase A2Activity during Zebrafish Embryogenesis,J Biol Chem, vol. 274, 19338-46 (1999).
Fang, X. et al, Lysophospholipid Growth Factors in the Initiation, Progression, Metastases, and Management of Ovarian Cancer,Ann NY Acad Sci, vol. 905, 188-208 (2000).
Feng, L. et al, A Real-Time Flurogenic Phospholipase A2Assay for Biochemical and Cellular Activity Measurements—Chemistry&Biology, vol. 9, 795-803 (2002).
Heise, C. et al, Activity of 2-Substituted Lysophosphatidic Acid (LPA) Analogs at LPA Receptors: Discovery of a LPA1/LPA3Receptor Antagonist,Mol Pharmacol, vol. 60, 1173-80 (2001).
Hendrickson, H. et al, Evaluation of Fluorescent and Colored Phosphatidylcholine Analogs as Sustrates for the Assay of Phospholipase A21, Analyt Biochem, vol. 185, 80-3 (1990).
Hla, T. et al, Lysophospholipids—Receptor Revelations,Science, vol. 294, 1875-8 (2001).
Imamura, F. et al, Induction of In Vitro Tumor Cell Invasion of Cellular Monolayers by Lysophosphatidic Acid or Phospholipase D,Biochem Biophy Res Commun, vol. 193, 497-503 (1993).
Luquain, C. et al, Lysophosphatidic acid signaling: how a small lipid does big things,Trends Biochem Sci, vol. 28, 377-83 (2003).
Mills, G. et al, A Putative New Growth Factor in Ascitic Fluid from Ovarian Cancer Patients: Identification, Characterization, and Mechanism of Action,Cancer Res, vol. 48, 1066-71 (1988).
Mills, G. et al, Critical Role of Lysophospholipids in the Pathophysiology, Diagnosis, and Management of Ovarian Cancer,Cancer Treat Res, vol. 107, 259-83 (2002).
Mills, G. et al, Future for Ovarian Cancer Screening: Novel Markers from Emerging Technologies of Transcriptional Profiing and Proteomics,J Natl Cancer Inst, vol. 93, 1437-9 (2001).
Moolenaar, W. et al, Lysophosphatidic acid: G-protein signalling and cellular responses,Curr Opin Cell Bio, vol. 9, 168-73 (1997).
Moolenaar, W., Bioactive Lysophospholipids and Their G Protein-Coupled Receptors,Exp Cell Res, vol. 253, 230-8 (1999).
Nam, S., et al, Autotaxin (ATX), a potent tumor motogen, augments invasive and metastatic potential of ras-transformed cells,Oncogene, vol. 19, 241-7 (2000).
Nieuwenhuizen, W. et al, Synthesis of a novel fluorescent ceramide analogue and its use in the characterization of recombinant ceramidase fromPseudomonas aeruginosaPA01,Chem Phys Lipids, vol. 114, 181-91 (2002).
Pankuch, J. et al, N-Me-pAB-Glu-y- Glu-y-Tyr(3-NO2): An Internally Quenched Fluorogenic y-Glutamyl Hydrolase Sustrate,Bioorg Med Chem Lett, vol. 11, 1561-4 (2001).
Sandro, S. et al, Quencher as Leaving Group: Efficient Detection of DNA-joining Reasctions,J Am Chem Soc, vol. 124, 2096-7 (2002).
Stassar, M. et al, Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization,Br J Cancer, vol. 85, 1372-82 (2001).
Tokumura, A. et al, Involvement of Iysophospholipase D in the production of Iysophosphatidic acid in rat plasma,Biochim Biophys Acta, vol. 875, 31-8 (1986).
Tokumura, A., Physiological and pathophysiological roles of lysophosphatidic acids produced by secretory lysophospholipase D in body fluids,Biochimica et Biophysica Acta, vol. 1582, 18-25 (2002).
Tokumura, A. et al, Identification of Human Plasma Lysophospholipase D, a Lysophosphatidic Acid-producing Enzyme, as Autotaxin, a Multifunctional Phosphodiesterase,J Biol Chem, vol. 277, 39436-42 (2002).
Tokumura, A. et al, Increased formation of lysophosphatidic acids by lysophospholipase D in serum of hypercholesterolemic rabbits,J Lipid Res, vol. 43, 307-15 (2002).
Tyagi, S. et al, Molecular Beacons: Probes that Fluoresce upon Hybridization,Nat Biotechnol, vol. 14, 303-8 (1996).
Umezu, M. et al, Autotaxin has lysophospholipase D activity leading to tumor cell growth and motility by lysophosphatidic acid production,Journal of Cell Biology, vol. 158, 227-33 (2002).
Xu, Y. et al, The Role and Clinical Applications of Bioactive Lysolipids in Ovarian Cancer,J Soc Gynecol Investig, vol. 8, 1-13 (2001).
Xu, Y. et al, Characterization of an Ovarian Cancer Activating Factor in Ascites from Ovarian Cancer Patients,Clin Cancer Res, vol. 1, 1223-32 (1995).
Yang, S. et al, Expression of autotaxin (NPP-2) is closely linked to invasiveness of breast cancer cells,Clin Exp Metastasis, vol. 19, 603-9 (2002).
Yang, Y. et al, Autotaxin Expression in Non-Small-Cell Lung Cancer,Am J Respir Cell Mol Biol, vol. 21, 216-22, (1999).
Zhang, G. et al, Expression of autotaxin mRNA in human hepatocellular carcinoma,Chin Med J(Engl), vol. 112, 330-2 (1999).
Zaikova, T. et al, Synthesis of Fluorogenic Substrates for Continuous Assay of Phosphatidylinositol-Specific Phospholipase C,Bioconjugate Chem, vol. 12, 307-13 (2001).
Ferguson Colin
Prestwich Glenn
Echelon Biosciences Incorporated
Hanley Susan
Marx Irene
McCallum Esq. Jennifer M.
The McCallum Law Firm, P.C.
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