Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1995-04-21
1997-08-19
Jacobson, Dian C.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
435212, 435226, 4353201, 435352, 435369, 435359, 536 232, C12N 510, C12N 948, C12N 1500, C12N 1558
Patent
active
056587880
ABSTRACT:
The present invention provides derivatives of tissue plasminogen activator that lack the Finger, Growth Factor and Kringle 1 domains and comprise a Kringle 2 domain that is monoglycosylated at a site other than that of t-PA. Using recombinant DNA techniques, an alternate glycosylation sequence is provided within the Kringle 2 domain of these t-PA derivatives. This alternate glycosylation consensus sequence, as well as the glycosylation consensus sequence within the Serine Protease domain, is glycosylated upon the expression and secretion of these molecules from eucaryotic host cells. Thus, a homogeneous population of diglycosylated t-PA derivatives that lack the Finger, Growth Factor and Kringle 1 domains is produced.
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Berg David T.
Grinnell Brian W.
Eli Lilly and Company
Jacobson Dian C.
Jones Joseph A.
Maciak Ronald S.
Plant Thomas G.
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