Compounds and methods for identifying activated platelets

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436503, 436800, 436811, 546 37, 546196, 546200, G01N 33567, C07D40114, C07D40106

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active

056460007

ABSTRACT:
The invention is directed to a series of novel compounds, and their pharmaceutically acceptable salts, of the formula ##STR1## wherein R is C.sub.0-6 alkyl substituted with R.sup.5 or a mono or polycyclic aromatic or heteroaromatic system comprised of 5 or 6-membered aromatic or heteroaromatic rings that are either unsubstituted or substituted with one or more of R.sup.1 and R.sup.2 ; R.sup.1 and R.sup.2 are independently C.sub.1-6 alkyl, carboxyl, hydroxyl, azido, nitro, amino, C.sub.1-6 alkylamino, C.sub.1-6 dialkylamino, arylamino, aryl C.sub.1-6 alkylamino, hydroxysulfonyl or arylazo; aryl is a phenyl or naphthyl ring which is unsubstituted or substituted with one or more of R.sup.3 and R.sup.4 ; R.sup.3 and R.sup.4 are independently C.sub.1-6 alkyl, azido, nitro, amino, C.sub.1-6 dialkylamino or hydroxysulfonyl; R.sup.5 is ##STR2## provided that when R is an unsubstituted monocyclic ring, the monocyclic ring is not phenyl or pyridyl. Such compounds are useful as fluorescent probes for identifying antiplatelet agents which selectively bind to activated platelets.

REFERENCES:
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patent: 5334596 (1994-08-01), Hartman et al.
patent: 5389631 (1995-02-01), Claremon et al.
D'Souza, "Platelet activation and arterial thrombosis", The Lancet, Oct. 1994, vol. 344, pp. 991-995.
Glusko et al. "Fibrinogen Receptors in Platelet Adhesion to Surfaces of Extracorporeal Circuit", J. Physiol. 1987, 252:H, pp. 615-621.

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