Compounds and methods for determination of thiols

Details Compounds and methods for determination of thiols Compounds and methods for determination of thiols

1998-05-15

2000-08-22

McKane, Elizabeth

Chemistry: analytical and immunological testing

Sulfur containing

436120, 436164, 422 56, G01N 3352

Patent

active

061071005

ABSTRACT:
Compositions and methods are provided for determining thiols or disulphides in which a sulfur containing analyte is combined with a thiol-specific reagent to produce an intermediate, and the intermediate is combined with a solvent insoluble compound to produce a solid state complex. In one aspect of preferred embodiments the intermediate comprises a chromophore, and in especially preferred embodiments the chromophore is detectable with the naked eye. In another aspect of preferred embodiments the thiol-specific reagent comprises a nitroso-donor, and more preferably Na-nitroprusside. In yet another aspect of preferred embodiments, the solvent insoluble compound comprises a basic or acidic metallic compound, or a polymeric matrix. Still more preferred solvent insoluble compounds comprise Zn-hydroxide, Ni-hydroxide or Al-hydroxide. These compositions and methods are more sensitive and reliable method than that previously available, especially for the determination of homocysteine.

REFERENCES:
patent: 4414414 (1983-11-01), Novak
patent: 4543336 (1985-09-01), Westup
patent: 5149661 (1992-09-01), Gjerde et al.
patent: 5397710 (1995-03-01), Steinman
Leeuwenkamp et al. Specific cation effect in the reaction of nitroprusside with cysteine, acetophenone and sulfite (Legal and Boedeker reaction), Pharmaceutisch Weekblad Scientific Edition, vol. 6, pp. 195-202, 1984.
Leeuwenkamp, O.R. et al. "Specific cation effect in the reaction of nitroprusside with cysteine, acetophenone and sulfite (Legal and Boedeker reaction)", Pharm. Weekbl., Sci. Ed., 6(5), pp. 195-202, 1984.

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