Compound water-insoluble glucan and process for the production t

Details Compound water-insoluble glucan and process for the production t Compound water-insoluble glucan and process for the production t

1976-12-10

1978-02-07

Jones, Raymond N.

Textiles: fiber preparation

195 31P, 195 96, 536 1, C12D 1304, C07G 300, C08B 3700

Patent

active

040725677

ABSTRACT:
A culture medium composed of carbon source, nitrogen source and minerals was sterilized at 120.degree. for 15 minutes, inoculated with strains of the genus Streptococcus and then cultivated at 35.degree. C for 72 hours under aeration and agitation. Subsequent to the cultivation, the resultant was subjected to water-washing and sieving to obtain crude glucan. The crude glucan was then dissolved in NaOH solution and centrifuged. The recovered filtrate was neutralized with HCl and then the insolubilized glucan was collected by centrifugation. The collected glucan was washed with water thoroughly until no chloride ion was detected in the waste water and then dried to form white purified glucan powder.

REFERENCES:
ebisu et al., "Studies on the Structures of Polysaccharides (Glucans and Fructans) Produced by Cariogenic Streptococci and on an Enzyme Hydrolyzing the Insoluble Glucan. I. Structural Studies of Insoluble Glucan, Soluble Glucan, and Fructans," Chemical Abstracts, vol. 85, No. 21, pp. 235, 236 (1976), Abs. No. 156181a.
Ebisu et al., "The Structure of Water-Insoluble Glucans of Cariogenic Streptococcus Mutans, Formed in the Absence and Presence of Dextranase," Carbohydrate Research, vol. 38 (1974), pp. 374-381.
Baird et al., "Investigation of the Polysaccharides Produced by Extracellular Glycosyltransferases from Streptococcus Mutans," Chemical Abstracts, vol. 77, No. 11, p. 242 (1972), Abs. No. 72399z.
Jeanes et al., "Characterization and Classification of Dextrans from Ninety-Six Strains of Bacteria," J. Amer. Chem. Soc., vol. 76, No. 20, (1954), pp. 5041-5052.

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