Compositions useful in anaerobic determination of analytes

Details Compositions useful in anaerobic determination of analytes Compositions useful in anaerobic determination of analytes

1994-05-06

1995-07-18

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 4, 435 25, 435 26, 435 14, C12Q 160, C12Q 154, C12Q 126, C12Q 100

Patent

active

054340551

ABSTRACT:
The invention describes a composition useful in determining sample analytes, where the determination is carried out anaerobically. The compositions include an analyte oxidizing agent, an electron transfer agent, ferric ions, and two chelators. The first chelator complexes to ferric ions, but does not have good affinity for ferrous ions. The second chelator does chelate ferrous ions, and forms a colored complex with the ion. It is the colored complex which serves as the indicator for the analyte. Different formulations of the composition are described.

REFERENCES:
patent: 4576913 (1986-03-01), Adachi et al.
patent: 4701420 (1987-10-01), Thunberg et al.
patent: 4912035 (1990-03-01), Belly et al.
patent: 4929544 (1990-05-01), Freitag
patent: 5141855 (1992-08-01), Schmittou
Al-Jobore et al; Chem. Abstract 94(1): 1872p from Can. J. Biochem., 58(12), 1397-1404 (1980).

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