Compositions of proteus vulgaris chondroitinase I and chondroiti

Details Compositions of proteus vulgaris chondroitinase I and chondroiti Compositions of proteus vulgaris chondroitinase I and chondroiti

1995-04-24

1998-02-10

Weber, Jon P.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

424 9461, 435200, 435201, 435202, A61K 3846, A61K 3847, C12N 924, C12N 926, C12N 928

Patent

active

057166170

ABSTRACT:
Chondroitinase II, a protein having an isoelectric point of approximately 8.4-8.45 and an apparent molecular mass of 112 kDa when electrophoresed in a 4 to 20% gradient acrylamide gel in 25 mM Tris/192 mM glycine buffer at pH 8.5 in the presence of about 0.1% (w/v) SDS, has been isolated and purified. A process for the copurification by affinity chromatography of the chondroitinase I and chondroitinase II proteins produced by Proteus vulgaris is also provided. The proteins can be further purified by metal chelating chromatography. Therapeutic or surgical compositions of isolated chondroitinases I and II or the copurified mixture of chondroitinase I and II are also disclosed. These compositions are used in a method for selectively and completely disinserting the vitreous body from the neural retina of an eye.

REFERENCES:
patent: 4696816 (1987-09-01), Brown
patent: 5049501 (1991-09-01), Katsuragi et al.
patent: 5198355 (1993-03-01), Kikuchi et al.
patent: 5292509 (1994-03-01), Hashimoto et al.
patent: 5498536 (1996-03-01), Khandke
patent: 5525500 (1996-06-01), Khandke et al.
Salyers et al. (Aug. 1988), Applied & Environmental Microbiology, 54 (8): 1964-1969.
Glover, D.M., (1985), "DNA Cloning, vol. 1, A Practical Approach", pp. 49-77, IRL Press (Oxford).
Scopes (1982) Protein Purification. New York: Springer Verlag. pp. 197-199.
Guthrie et al., J. Bacteriology 164:510-515.
Studier et al. (1990), Methods in Enzymology 185:60-85.
Lathe (1985), J. Mol. Biol. 183:1-11.
Bowie et al. (ed.) (1990), Science 247:1306-1310.
Demain et al. Manual of Industrial Microbiology and Biotechnology. Washington D.C.:American Society for Microbiology, 1986, pp. 154-169.
Kitamikado et al., Applied Microbiology, 29:414-421, 1975.
Sato et al., Applied Microbiology Biotechnol., 41:39-46, 1994.
Sato et al., Agric. Biol. Chem., 50:1057-1059, 1986.
Yamagata et al. J. Biol. Chem., 243:1523-1535, 1968.

Affiliated with

Also associated with

Rating

Compositions of proteus vulgaris chondroitinase I and chondroiti does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Compositions of proteus vulgaris chondroitinase I and chondroiti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions of proteus vulgaris chondroitinase I and chondroiti will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2075006