Chemistry: electrical and wave energy – Apparatus – Electrophoretic or electro-osmotic apparatus
Patent
1997-05-08
1999-09-07
Redding, David A.
Chemistry: electrical and wave energy
Apparatus
Electrophoretic or electro-osmotic apparatus
204603, 204604, G01N 2726
Patent
active
059482311
ABSTRACT:
Compositions, methods, and apparatus for performing ultrafast binding assays by capillary electrophoresis or other electroseparation techniques are disclosed. In one embodiment, a first binding partner carries a detectable label and a second binding partner is modified to be highly charged. When used in combination with a sample containing an analyte with which both binding partners can interact and bind thereto, a three-membered complex is formed. The electrophoretic mobility difference between the unbound and complex-bound forms of labeled first binding partner is such that electroseparation and subsequent detection of an analyte can be accomplished. The compositions, methods, and apparatus disclosed herein also permit quantitative determination of the concentration of an analyte in a sample.
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Fuchs Martin
Nashabeh Wassim A.
Schmalzing Dieter R.
PerSeptive Biosystems Inc.
Redding David A.
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