Compositions, methods and apparatus for ultrafast electroseparat

Details Compositions, methods and apparatus for ultrafast electroseparat Compositions, methods and apparatus for ultrafast electroseparat

1995-04-20

1997-05-20

Schain, Howard E.

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204603, 204604, 204451, 204452, 204453, C25B 700

Patent

active

056309244

ABSTRACT:
Compositions, methods, and apparatus for performing ultrafast binding assays by capillary electrophoresis or other electroseparation techniques are disclosed. In one embodiment, a first binding partner carries a detectable label and a second binding partner is modified to be highly charged. When used in combination with a sample containing an analyte with which both binding partners can interact and bind thereto, a three-membered complex is formed. The electrophoretic mobility difference between the unbound and complex-bound forms of labeled first binding partner is such that electroseparation and subsequent detection of an analyte can be accomplished. The compositions, methods, and apparatus disclosed herein also permit quantitative determination of the concentration of an analyte in a sample.

REFERENCES:
patent: 4329213 (1982-05-01), Elwing
patent: 4486530 (1984-12-01), David et al.
patent: 5120413 (1992-06-01), Chen et al.
patent: 5122248 (1992-06-01), Karger et al.
patent: 5137609 (1992-08-01), Manian et al.
patent: 5348633 (1994-09-01), Karger et al.
Schmalzing et al. (1995), "Rapid Capillary Electrophoresis Based Immunoassays," Abstract of presentation given at 1995 AACC Oak Ridge Conference, San Antonio, TX, Apr. 28, 1995.
Nielsen et al. (1991), "Separation of Antibody-Antigen Complexes By Capillary Zone Electrophoresis, Isoelectric Focusing and High-Performance Size-Exclusion Chromatography," 539 J. Chromatography 177-185.
Schultz et al. (1993), "Rapid Immunoassays Using Capillary Electrophoresis With Fluorescence Detection," 65 Anal. Chem. 3161-3165.
Shimura et al. (1994), "Affinity Probe Capillary Electrophoresis: Analysis of Recombinant Human Growth Hormone With A Fluorescent Labeled Antibody Fragment," 55 Anal. Chem. 9-15.
Chen et al. (1994), "Characterization of Proteins By Capillary Electrophoresis in Fused-Silica Columns: Review On Serum Protein Analysis and Application to Immunoassays," 15 Electrophoresis 13-21.
Chen et al. (1994), "Feasibility Studies for Simultaneous Immunochemical Multianalyte Drug Assay by Capillary Electrophoresis with Laser-Induced Fluorescence," 40 Clin. Chem. 9:1819-1822.
Raymond et al. (1994), "Continuous Sample Pretreatment Using A Free-Flow Electrophoresis Device Integrated Onto A Silicon Chip", 66 Anal. Chem. 2858-2865.
Reif et al. (1994), "Fluorescein Isothiocyanate-Labeled Protein G As An Affinity Ligand In Affinity/Immunocapillary Electrophoresis With Fluorescence Detection," 66 Anal. Chem. 4027-4033.
Evangelista et al. (1995), "Simultaneous Multianalyte Immunoassay of Drugs of Abuse by CE," 14 Amer. Clin. Lab. 2:27-28.
Egholm et al. (1993), "PNA Hybridizes to Complementary Oligonucleotides Obeying the Watson-Crick Hydrogen-Bonding Rules," 365 Nature 566-568.
Chen et al. (1994), "Characterization of Charge-Modified and Fluorescein-Labeled Antibody by Capillary Electrophoresis Using Laser-Induced Fluorescence," 680 J. Chromatography 2:419-423.

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