Compositions for the prevention and treatment of verotoxin-induc

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus

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424 934, 424 9348, 4242411, A01N 6300, A61K 39108

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active

060804009

ABSTRACT:
The present invention includes methods for generating neutralizing antitoxin directed against verotoxins. In preferred embodiments, the antitoxin directed against these toxins is produced in avian species using soluble recombinant verotoxin proteins. This antitoxin is designed so as to be administrable in therapeutic amounts and may be in any form (i.e., as a solid or in aqueous solution). These antitoxins are useful in the treatment of humans and other animals intoxicated with at least one bacterial toxin, as well as for preventive treatment, and diagnostic assays to detect the presence of toxin in a sample.

REFERENCES:
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K. Ramotar et al. Characterization of Shiga-like toxin I B subunit purified from overproducing clones of the SLT-I B cistron. Biochem. J. 272: 805-811, 1990.
TM Zollman et al. Purification of recombinant Shiga-like toxin type I A1 fragment from E. coli. Protein Expression and Purification 5: 291-295, 1994.
SB Calderwood et al. A system for production and rapid purification of large amounts of the Shiga toxin/Shiga-like toxin I B subunit. Infect. Immun. 58: 2977-2982, 1990.
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LP Perera et al. Infect. Immun. 59: 829-835, 1991.

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