Compositions for the detection of proteases in biological sample

Details Compositions for the detection of proteases in biological sample Compositions for the detection of proteases in biological sample

1994-10-28

1997-02-25

Hollinden, Gary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 76, 435 772, 435 79, 435 18, 436 86, 436 89, 436 74, 436800, 436172, 530300, C12Q 100, C12Q 137, G01N 3300

Patent

active

056058098

ABSTRACT:
The present invention provides for novel reagents whose fluorescence increases in the presence of particular proteases. The reagents comprise a characteristically folded peptide backbone each end of which is conjugated to a fluorophore. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. Because of their high fluorescence signal in the visible wavelengths, these protease indicators are particularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections. Thus this invention also provides for methods of detecting protease activity in situ in frozen sections.

REFERENCES:
patent: 4557862 (1985-12-01), Mangel et al.
patent: 4648893 (1987-03-01), Roux
patent: 4708929 (1987-11-01), Henderson
patent: 4780421 (1988-10-01), Kameda et al.
patent: 4897444 (1990-01-01), Brynes et al.
patent: 5212298 (1993-05-01), Rademacher et al.
Carmel, A. et al. FEBS Letters, 30:11 (1973).
Knight, C. G. et al. FEBS 10610, 296:263 (1992).
Latt, S. A. et al. Analytical Biochemistry 50:56 (1972).
Matayoshi, E. D. et al. Science, 247: (1990).
Nagase, H. et al. The Journal of Biological Chemistry 269:20952 (1994).
Wang, G. T. et al. Tetrahedron Letters 31:6493 (1990).
Wu, P. et al. Analytical Biochemistry 218:1 (1994).

Affiliated with

Also associated with

Rating

Compositions for the detection of proteases in biological sample does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Compositions for the detection of proteases in biological sample, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions for the detection of proteases in biological sample will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1973514