Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues
Reexamination Certificate
2005-08-30
2005-08-30
Weber, Jon (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
C530S324000, C530S326000, C514S002600, C514S013800, C435S023000, C435S024000, C435S007720
Reexamination Certificate
active
06936687
ABSTRACT:
The present invention provides for novel reagents whose fluorescence increases in the presence of particular proteases. The reagents comprise a characteristically folded peptide backbone each end of which is conjugated to a fluorophore. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. Because of their high fluorescence signal in the visible wavelengths, these protease indicators are particularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections. Thus this invention also provides for methods of detecting protease activity in situ in frozen sections.
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Komoriya Akira
Packard Beverly S.
Hunter Tom
Kam Chih-Min
Onco Immunin, Inc.
Quine I. P. Law Group, P.C.
Weber Jon
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