Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-06-07
1997-08-05
Elliott, George C.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 6, 4353201, 536 242, C12N 1509, C12Q 168, C07H 2100
Patent
active
056544138
ABSTRACT:
The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each consist of a plurality of subunits 3 to 6 nucleotides in length selected from a minimally cross-hybridizing set. A subunit of a minimally cross-hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports. This embodiment provides a readily automated system for manipulating and sorting polynucleotides, particularly useful in large-scale parallel operations, such as large-scale DNA sequencing, mRNA fingerprinting, and the like, wherein many target polynucleotides or many segments of a single target polynucleotide are sequenced simultaneously.
REFERENCES:
patent: 5302509 (1994-04-01), Cheeseman
patent: 5482836 (1996-01-01), Cantor et al.
PCT/US91/09217 WO92/10587 Dower et al. Sequencing of surface immobilized polymers utilizing microfluorescence detection Jun. 25, 1992.
PCT/US93/03418 WO93/21203 Shortle et al. Synthesis of diverse and useful collections of oligonucleotides Oct. 28, 1993.
PCT/US93/09345 WO94/08051 Still et al. Complex combinatorial chemical libraries encoded with tage Apr. 14, 1994.
PCT/US93/03767 WO 93/22680 McGall et al. Spacially-addressable immobilization of oligonucleotides and other biological polymers on surfaces Nov. 11, 1993.
PCT/US91/09226 WO92/10588 Fodor et al. Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides Jun. 25, 1992.
PCT/US93/04145 WO 93/22684 Fodor et al. Factorial Chemical Libraries Nov. 11, 1993.
PCT/US91/04666 WO 92/00091 Lam et al. Random Bio-oligomer library, a method of synthesis thereof, and a method of use thereof Jan. 9, 1992.
PCT/GB89/01114 WO 90/03382 Southern et al. Support-bound oligonucleotides Apr. 5, 1990.
PCT/GB95/00109 WO95/20053 Sibson Sequencing of Nucleic Acids Jul. 27, 1995.
PCT/US92/07815 (WO93/06121) Dower et al. Method of synthesizing diverse collections of oligomers Apr. 1, 1993.
European patent appl. 90107066.4 (Publ. No. 0 392 546 A2) Drmanac and Crkvenjakov Process for determination of a complete or a partial contents of very short sequences in the samples of nucleic acids connected to the discrete particles of microscopic size by hybridization with oligonucleotide probes Oct. 17, 1990.
0303459A3 Church Mulitplex sequencing Feb. 15, 1989.
Canadian Patent Application 2,036,946 Deugau et al. Indexing linkers. Oct. 7, 1991.
Crick et al, "Codes without commas," Proc. Natl. Acad. Sci., 43: 416-421 (1957).
Ohlmeyer et al, "Complex synthetic chemical libraries indexed with molecular tags," Proc. natl. Acad. Sci., 90: 10922-10926 (1993).
Maskos and Southern, "Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesized in situ," Nucleic Acids Research, 20: 1679-1684 (1992).
Matthews and Kricka, "Analytical strategies for the use of DNA probes," Anal. Biochem. 169: 1-25 (1988).
Broude et al., "Enhanced DNA sequencing by hybridization," Proc. Natl. Acad. Sci. 91: 3072-3076 (1994).
Nielsen et al., "Synthesis methods for the implementation of encoded combinatorial chemistry," J. Am. Chem. Soc. 115: 9812-9813 (1993).
Needels et al, "Generation and screening of an oligonucleotide-encoded synthetic peptide library," Proc. Natl. Acad. Sci., 90: 10700-10704 (1993).
Chetverin et al, "Oligonucleotide arrays: New concepts and possibilities," Biotechnology, 12: 1093-1099 (1994).
Yang and Youvan, "A prospectus for multipspectral-multiplex DNA sequencing," Biotechnology, 7: 576-580 (1989).
Church et al, "Multiplex DNA Sequencing," Science, 240: 185-188 (1988).
Beck et al, "A strategy for the amplification, purification, and selection of M13 templates for large-scale DNA sequencing," Analytical Biochemsitry, 212: 498-505 (1993).
Ji and Smith, "Rapid purification of double-stranded DNA by triple-helix-mediated affinity capture," Anal. Chem., 65: 1323-1328 (1993).
Brown et al, "A new base-stable linker for solid-phase oligonucletide synthesis," J. Chem. Soc. Commun. 1989: 891-893.
Oliphant et al, "Cloning of random-sequence oligodeoxynucleotides," Gene, 44: 177-183 (1986).
Hunkapiller et al, "Large-scale and automated DNA sequence determination," Science, 254: 59-67 (1991).
Coche et al, "Reducing bias in cDNA sequence representation by molecular selection," Nucleic Acids Research, 22: 4545-4546 (1994).
Kuijper et al, "Functional cloning vectors for use in directional cDNA cloning using cohesive ends produced with T4 DNA polymerase," Gene, 112: 147-155 (1992).
Aslanidis et al, "Ligation-independent cloning of PCR products (LIC-PCR)," Nucleic Acids Research, 18: 6069-6074 (1990).
Wetmur, "DNA probes: applications of the principles of nucleic acid hybridization," Critical Reviews in Biochemistry and Molecular Biology, 26: 227-259 (1991).
Egholm et al, "PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules," Nature, 365: 566-568 (1993).
Gryaznov et al, "Modulation of oligonucleotide duplex and triplex stability via hydrophobic interactions," Nucleic Acids Research, 21: 5909-5915 (1993).
Brenner et al., "Encoded combinatorial chemistry", Proc. Natl. Acad. Sci. USA, 89: 5381-5383. Jan. 1992.
Elliott George C.
Macevicz Stephen C.
Priebe Scott D.
Spectragen, Inc.
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