Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2008-03-04
2008-03-04
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Vector, per se
Reexamination Certificate
active
10425529
ABSTRACT:
A method, vector, and kit are provided for selectively isolating genes encoding membrane-bound proteins by fusing proteins to a secretable protein having a binding affinity to an antigen (e.g., an antibody), expressing these fusions in host cells, and selectively isolating individual host cells by their ability to bind the antigen via the fusion protein. Host cells expressing fusion proteins that do not contain a membrane binding domain will be relatively unable to bind the antigen, since the fusion proteins in those cells are secreted and unattached to their host cells. The method, vector, and kit disclosed herein are particularly useful for identifying genes for membrane-bound proteins represented in cDNA libraries.
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Ohtomo Toshihiko
Saito Mikiyoshi
Tsuchiya Masayuki
Chugai Seiyaku Kabushiki Kaisha
Fish & Richardson P.C.
Horlick Kenneth R.
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