Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2008-03-04
2008-03-04
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Vector, per se
Reexamination Certificate
active
07338797
ABSTRACT:
A method, vector, and kit are provided for selectively isolating genes encoding membrane-bound proteins by fusing proteins to a secretable protein having a binding affinity to an antigen (e.g., an antibody), expressing these fusions in host cells, and selectively isolating individual host cells by their ability to bind the antigen via the fusion protein. Host cells expressing fusion proteins that do not contain a membrane binding domain will be relatively unable to bind the antigen, since the fusion proteins in those cells are secreted and unattached to their host cells. The method, vector, and kit disclosed herein are particularly useful for identifying genes for membrane-bound proteins represented in cDNA libraries.
REFERENCES:
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5468614 (1995-11-01), Fields et al.
patent: 5474899 (1995-12-01), Lisi
patent: 5525486 (1996-06-01), Honjo et al.
patent: 5571894 (1996-11-01), Wels et al.
patent: 5817308 (1998-10-01), Scott et al.
patent: 5858752 (1999-01-01), Seed et al.
patent: 6030834 (2000-02-01), Chu et al.
patent: 6610485 (2003-08-01), Tsuchiya et al.
patent: 6613746 (2003-09-01), Seed et al.
patent: 2097416 (1992-06-01), None
patent: 2120153 (1993-04-01), None
patent: 2329986 (1999-11-01), None
patent: 0939119 (1999-09-01), None
patent: 11-32779 (1999-09-01), None
patent: WO 96/40904 (1996-12-01), None
patent: WO 98/03645 (1998-01-01), None
Kojima et al., “A signal sequence trap based on a constitutively active cytokine receptor”, Nature Biotech., 17:487-490, 1999.
Tashiro et al., “Signal Sequence Trap: A Cloning Strategy for Secreted Proteins and Type I Membrane Proteins”, Science, 261:600-603, 1993.
Kelley, et al., “Functional Significance and Evolutionary Development of the 5′-Terminal Regions of Immunoglobulin Variable-Region Genes”, Cell, vol. 29(2), pp. 681-689 (1992).
Thibault, et al., “Characterization and Biologic Activities of Recombinant Rat Soluble Interleukin-6 Receptor”, Journal of Interferon and Cytokine Research, vol. 16(11), pp. 973-981 (1996).
Lord, et al., “Leukemia Inhibitory Factor and Interleukin-6 Trigger the Same Immediate Early Response, Including Tyrosine Phosphorylation, upon Induction of Myeloid Leukemia Differention”, Molecular and Cellular Biology, vol. 11(9), pp. 4371-4379 (1991).
Miyoshi et al., “A Transmembrane Trap Method for Efficient Cloning of Genes Encoding Proteins Possessing Transmembrane Domain,” Biochem. Biophysical Research Communications, vol. 289, No. 5, pp. 1192-1198 (2001).
Sugano et al., “Transmembrane-Domain Trapping: A Novel Method for Isolation of cDNAs Encoding Putative Membrane Proteins,” DNA Research, vol. 5, No. 3, pp. 187-193 (1998).
Rosanne D. Dunn et al. “Antigen binding and cytotoxic properties of a recombinant immunotoxin incorporating the lytic peptide, melittin”. Immunotechnology 2:229-240, 1996.
Susanna M. Rybak et al. “Humanization of immunotoxins”. Proc. Natl. Acad. Sci. USA89:3165-3169, Apr. 1992.
Forster et al. “Expression of Foreign Genes in Mammalian Cells Using an Antibody Fusion System”, Molecular Biotechnology 1:251-263, 1994.
Ohtomo Toshihiko
Saito Mikiyoshi
Tsuchiya Masayuki
Chugai Seiyaku Kabushiki Kaisha
Fish & Richardson P.C.
Horlick Kenneth R.
LandOfFree
Compositions for isolating a cDNA encoding a membrane-bound... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compositions for isolating a cDNA encoding a membrane-bound..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions for isolating a cDNA encoding a membrane-bound... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2791372