Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-09-28
1999-04-06
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
058916296
ABSTRACT:
The present invention addresses compositions and methods for cleaving nucleic acids. The invention allows one to reliably detecting point mutations in long and short target regions of nucleic acids in a safe, non-labor intensive, and cost effective manner. The methods and compositions of the present invention allow for the use of various RNases, such as RNase A, RNase I and RNase T2, in the detection of mutations. The present invention also identifies reaction conditions that result in significant improvement in specific mismatch cleavage in the NIRCA.TM. assay.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4946773 (1990-08-01), Maniatis et al.
patent: 5476774 (1995-12-01), Wang et al.
patent: 5571905 (1996-11-01), Vogelstein et al.
patent: 5576422 (1996-11-01), Vogelstein et al.
patent: 5582986 (1996-12-01), Monia et al.
patent: 5589329 (1996-12-01), Winkler et al.
patent: 5599673 (1997-02-01), Keating et al.
patent: 5602243 (1997-02-01), Vogelstein
Cristina et al., "Analysis of Genetic Variability in Human Respiratory Syncytial Virus by the RNase A Mismatch Cleavage Method: Subtype Divergence and Heterogeneity," Virology, 174:126-134, 1990.
Christina et al., "Evolution of the G and P Genes of Human Respiratory Syncytial Virus (Subgroup A) Studied by the RNase A Mismatch Cleaveage Method," Virology, 184:210-218, 1991.
Dunn et al., "Identification of Germline and Somatic Mutations Affecting the Retinoblastoma Gene", Science, 241:1797-1800, 1988.
Friedberg et al., "Selective Detection of mRNA Forms Encoding the Major Phenobarbital Inducible Cytochromes P450 and Other Members of the P45011B Family by the RNAse A Protection Assay", Archives of Biochemistry and Biophysics, 279:(1)167-173, 1990.
Genovese et al., "Detection of Mutations In Human Type I Collagen mRNA in Osteogenesis Imperfecta by Indirect RNase Protection", J. Biol. Chem., 264:(16)9632-9637, 1989.
Gibbs and Caskey, "Identification and Localization of Mutations at the Lesch-Nyhan Locus by Ribonuclease A Cleavage," Science, 236:303-305, 1987.
Gilman, "Ribonuclease Protection Assay", Current Protocols in Molecular Biology, Ausubel, F.N. et al., Ed., Unit 4.7:4.7.1-4.7.8, 1987, Mass. Gen. Hosp., Harvard Med. School, Boston, MA.
Goldrick, M., "Faster Mutation Analysis. Non-Isotopic Method Offers Advantages Over SSCP," Ambion TechNotes, 2(1):1-13, Ambion, Inc., Austin, Texas, 1995.
Kain et al., "Universal Promoter for Gene Expression Without Cloning: Expression-PCR", BioTechniques, 10:(3)366-373, 1991.
Kim et al., "Occurrence of p53 Gene Abnormalities in Gastric Carcinoma Tumors and Cell Lines", J. Natl. Cancer Res., 83:(13)938-943, 1991.
Kinzler et al., "Identification of a Gene Located at Chromosome 5q21 That is Mutated in Colorectal Cancers", Science, 251:1366-1370, 1991. Sensitive Detection of Differentially Spliced Substance P-and Neurokinin A-Encoding Messenger Ribonucleic Acids", Methods in Enzymology, 168:634-653.
Lopez-Galindez et al., "Characterization of genetic variation and 3'-azido-3' seoxythymidine-resistance mutations of human immunodeficiency virus by the RNase A mismatch cleavage method," Proc. Natl. Acad. Sci USA, 88:4280-4284, 1991.
Marquardt, et al., "Detection and localization of single-base sequence differences in foot-and-mouth disease virus genomes by the RNase mismatch cleavage method," Journal of Virological Methods, 33:267-282, May 1991.
Melton et al., "Efficient in vitro Synthesis of Biologically Active RNA and RNA Hybridization Probes for Plasmids Containing a Bacteriophage SP6 Promoter," Nucleic Acids Res., 12:7035-7056, 1984.
Myers et al., "Detection of Single Base Substitutions by Ribonuclease Cleavage at Mismatches in RNA:DNA Duplexes", Science, 230:1242-1246, 1985.
Myers et al., "Recent Advances in the Development of Methods for Detecting Single-base Substitutions Associated with Human Genetic Diseases", Cold Spring Harbor Symposia On Quantitative Biology, LI:275-284.
Nishisho et al., "Mutations of Chromosome 5q21 Genes in FAP and Colorectal Cancer Patients" Science, 253:665-669, 1991.
Perucho, "Detection of Single-base Substitutions with the RNAse A Mismatch Cleavage Method", Strategies in Molecular Biol., 2:(3)37-41, 1989.
Raines, R.T., "Structure, Mechanism and Function of Ribonucleases," Proceedings of the Second International Meeting, pp. 139-143, Universitat Autonoma de Barcelona, Bellaterra, Spain, 1991 (Cuchillo et al., eds.).
Resto et al., "Amplification of Protein Expression in a Cell Free System", Nucleic Acids Research, 20:(22)5979-5983, 1992.
Rosenzweig, "Preclinical Diagnosis of Familial Hypertrophic Cardiomyopathy by Genetic Analysis of Blood Lymphocytes", New Engl. J. Med., 325:(25)1753-1760, 1991.
Sambrook et al., "Molecular Cloning; Mapping Of RNA With Ribonuclease And Radiolabeled RNA Probes", Cold Spring Harbor Laboratory Press, 7.71-7.77, 1989.
Sarkar et al., "Access to a Messenger RNA Sequence or Its Protein Product Is Not Limited by Tissue or Species Specificity", Science, 244:331-334, 1989.
Stoflet et al., "Genomic Amplification with Transcript Sequencing", Science, 239:491-494, 1988.
Storch et al., "RNA Fingerprinting of Respiratory Syncytial Virus Using Ribonuclease Protection", J. Clin. Invest., 83:1894-1902, 1989.
Takahashi et al., "p53: A Frequent Target for Genetic Abnormalities in Lung Cancer", Science, 246:491-494, 1989.
Theophilus et al., "Comparison of RNase A, a chemical cleavage and GC-clamped denaturing gradient gel electrophoresis for the detection of mutations in exon 9 of the human acid b-glucosidase gene," Nucleic Acids Res., 17(19):7707-7722, 1989.
Ueda et al., "Detection of Multidrug Resistance (MDR1) Gene RNA Expression in Human Tumors by a Sensitive Ribonuclease Protection Assay", Jpn. J. Cancer Res., 80:1127-1132, 1989.
Watkins et al., "Characteristics and Prognostic Implications Of Myosin Missense Mutations In Familial Hypertrophic Cardiomyopathy", New Engl. J. Med. 326:(17)1108-1114, 1992.
Winter et al., "A Method to Detect and Characterize Point Mutations in Transcribed Genes: Amplification and Overexpression of the Mutant c-Ki-ras Allele in Human Tumor Cells", Proc. Natl. Acad. Sci. USA, 82:7575-7579, 1985.
Yang and Melera, "Application of the Polymerase Chain Reaction to the Ribonuclease Protection Assay," BioTechniques, 13(6)922-927, 1992.
International Search Report dated Jun. 27, 1996.
U.S. application No. 08/371,531, filed Jan. 9, 1995, Inventor Marianna Goldrick.
Almoguera et al., "Application of the Polymerase Chain Reaction for the Detection of Single-Base Substitutions by the RNase-A Mismatch Cleavage Method," pp. 37-45.
Ambion Inc.
Campbell Eggerton A.
LandOfFree
Compositions for improving RNase cleavage of base pair mismatche does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Compositions for improving RNase cleavage of base pair mismatche, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Compositions for improving RNase cleavage of base pair mismatche will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1370026